大豆雄性不育恢复育性相关基因aflp差异片段的筛选克隆和scar转换作物栽培学专业论文.docxVIP

突变情况。3)序列测定特异片段长度为3 突变情况。 3)序列测定特异片段长度为3 10bp，与网上以发表的序列同源 性比较，发现与大豆的GGMPM9有一部分的同源性，与其它发表的 序列同源性都很小，结果表明m10．3是恢复株群中发现的新的特 异的片段。 ～， 4)以特异片段为基础，设计引物进行了SCAR转换研究，将 一 AFLP标记成功的转化成稳定的SCAR标记。 关键词：大豆；雄性不育；恢复基因；SCAR；Ⅷ10一3 AbstractSoybean Abstract Soybean is a typical plant of self-pollinate，aIld it is an import扣1t resourse of seeds．As one of the maior crops in China aIld all over t11e、Ⅳorld，soybean plavs very impon乏mt role in world food supply a11d仃ade market．Utilization of heterosis is the iInpon锄t aIld useml way to promote crop output锄d quali戗One of the obstacle to utilize soybean heterosis is tllat moSt of tlle alien cytoplaSm has h觚nml e岱：ct锄d h嬲no excellent restoreing．Male sterili田、)l，ith cytoplaSm haS been proved to be promising in hybrid soybeaIl breeding，which has broad spectacle of reSto血g gene resourCes锄d haS no distinct harmful cytoplaSmic efI’ect．While吐lere are less restoring lines 诵tll botll higher restoI：ing abilit)，and excellent agrollomic pe墒肌aIlce in soybeaIl，so it is impossible to use them directly in hybrid production． Breeding of restorer is one of t11e prerequisite to produce hybrids using CMS system， and is more dimcult than conventional breedin＆because me presence of restoring genes in breeding lines callrlot be detected through仃aditional approaches、Ⅳithout a generation of progeny testing，、Ⅳhich is lab aIld time-consuming to cross eveD，plant of a segregating population wi也male sterile line，so the pl孤t witll excellent agronomic n面ts o舭n haS n0 Rf gene锄d the restomg ability gets stable slowly 廿lrou曲generations． The utilization of DNA molecular markers will be beneficial to overCome tlle dimcult)r of restorer breeding．Witll molecular markers，it is possible to detect segregatillg plants“ng seedling stage、航tllout plenty of test crossir培aIld eX锄iIlir培work，make the trait of restoring abili够get sta．ble quickly’raise accuracy in selecting restorations，pyr锄id minor RF genes，rajse resto血g ability aIld apply it to the m觚agement of hybrid seeds．At the sa