氟喹诺酮类药物与多西环素联用对牛源大肠杆菌的抑菌效果的应用兽医临床专业论文.docxVIP

CONTENTSCoNTENTS CONTENTS CoNTENTS Abstract in Chinese ．．．． ．． ． ．．． ． I Abstract in English ．． ．． ． ．． ． ．．． ．1II 1．Introduction 一1 1．1 Escherichia coli epidemic status 1 1．1．1 Classification and characteristics ofE．coli ． ．．．．． ． ．．．．． 1 1．1．2 Common E．coli disease ．．2 1．1．3 Status of Escherichia coli antimicrobial resistance 2 1．2 Mutant selection window ．． ．．．．． ．．．． ．．．．． ．． ． ．．．． 4 1．2．1 The establishment and development of the mutant selection window．．．． ．．． ．．．． ． ．．4 1．2．2 Progress MSW at home and abroad ．．．． ．． ．． ．．．．．．．． ．．．．． ．．． ．．．．．． ．．．．．5 1．3 Quinolone antibiotics ．．． ． ． ．．．． ．． ． ．．．．．． ．．．．． ．． ．． ．．7 1．3．1 Classification and development of quinolone antibiotics．．．． ．． ．．． ．． ．．． ． ． ．．．．7 1．3．2 Antibacterial mechanism．．．． ． ．．．．． ．．．．． ．．．．．．．．．． ． ．．．．． ．．． ．．．． 。7 1．3．3 Ofloxacin and ciprofloxacin ．．． ．．． ．．．．．．．． ．． ．．．．．． ．．．． ．．．．． 8 1．4 Tetracyclines antibiotics 8 1．4．1 Tetracyclines classification ．8 1．4．2 Resistance mechanisms． ． ．．． ．．．．． ．． ．．． ．． ．9 1．4．3 Doxycycline ．9 1．5 Drug combination ．． ．． ．．．． ．．． ．．．． ．． ．．．9 1．6 Investigative objective ．． ．． ． ． ．．．． ．．． ．．．．． ．． 一1 0 2．Experimental materials and methods ．1 1 2．1 Materials 1 1 2．1．1 Reagent and experimental instrument 一1 1 2．2 Methods ．． ． ．． ．． ． ．． ．． ．．．． 1 1 2．2．1 PCR for Escherichia coli from caries ．．． ．． ．． ．． ．12 2．2．2 Kirby-Bauer test ．． ． ．．．． ．．．1 2 2．2．3 MIC of strains ． ．． ． ． ． ．．．．1 2 2．2．4 MPC of strmns ． ． ． ．． ．． ．．． 。1 3 2．2．5 Drug combination ． ． ．． 14 2．2．6 Effect of drug concentration against colony numbers of anastate growth for mutant strains ． ． ． ．． ． ．．14 2．2．7 Different concentrations mutation detection 1 4 3．Results and analysis ． 1 6 3．1 The resultS of PCRfor Escheriehia coli from caries ．1 6 III 万方数据 东北农业大学兽医硕士学位论文3．2 东北农业大学兽医硕士学