反复熔铸对镍铬烤瓷合金生物相容性影响的研究口腔临床医学专业论文.docxVIP

Effect Effect of Recasting on Biocompability of the Nickel—Chromium Ceramic Alloy 【Abstract] objeetive：To study the effect of recasting in vitro cytotoxicity, haemolysis and oral mucous membrane irritmion test of the Nickel—Chromium Ceramic Alloy． Methods：(1)According to ISO 1 0993—5：1 999 standards，the Nickel—Chr- 一omium Ceramic Alloy was recasted by 1 to 5 times to make cylinder test specimens with 4．0cm diameter and 8．0mm high，then in vitro cytotoxicity test(MTT method)on L一929 cell were carried on to evaluate the cytotoxicity of each specimen．And analysi s the results between the specimens which were recasted 2 to 5 times with the specimens which were casted by 1 time． (2)According to ISO／TR 7405—1 984 standards，Nickel-Chromium Ceramic Alloy was recasted by 1 to 5 time to make the wafer test specimens with 5．0mm diameter and 1．0mm thick，and to test the haemolysis of each specimen，then to analysis the absorbance between the specimens which were recasted 2 to 5 times and the specimens which were casted by 1 time．(3)According to the ISO／TR 7405—1 984 standards，Nickel—Chromium ceramic alloy was recasted into wafer with 5．0mm diameter and O．5mm thick by 1％2-，3一，4一，and 5-time，the wafer were fixed to the oral mucous of golden hamster,then observe the changes of oral mucous membrane after 1 4 days． Results：(1)The level of cytotoxicity of all the experimental groups were ranged from 0 to 1．No statistically differences were found on the absorbance in different leaching liquid，between the specimens fabricated by 2一，3-,4-,and 5-time casting and the 1-time casting specimens(p0．05)．(2)Haemolysis rates of the recasted five times Nickel-Chromium ceramic alloy were 1．264％，1．205％，1．1 70％，1．300％，1．323％．No statistically differences were found on the absorbance between the specimens fabricated by 2-，3-,4-,and 一3一 5-time 5-time casting and the 1-time casting specimens(p0．05)．(3)There was no abnormal reaction on local mucous membrane such as hyperaemia， haemorrhage，erosion，or