用γ射线和叠氮化钠诱变的玉米愈伤组织筛选耐旱和雄性不.pdf

356 核 农 学 报 　2005 ,19 (5) :356～359 Acta Agriculturae N ucleatae Sinica ( ) 文章编号 2005 0535604 用γ射线和叠氮化钠诱变的玉米愈伤组织筛选 耐旱和雄性不育材料 付凤玲 　李晚忱 　荣廷昭　潘光堂 　谭登峰 ( 四川农业大学玉米研究所 , 四川 雅安 　625014) 摘 　要 : 60 γ ( ) 用 Co 射线和叠氮化钠 NaN3 处理玉米愈伤组织 ,在含 10 %NaCl 的高渗培养基上筛 选 ,对再生植株株系进行耐旱性鉴定 。结果表明 ,20 Gy 的辐射和 1 mmolL NaN3 是较为适合玉 米愈伤组织诱变的处理组配 。22 个再生植株株系中 ,有 5 个诱变株系的耐旱性高于未诱变对 照 ,其中 1 个株系的耐旱性与耐旱自交系“81565 ”接近 。此外 ,从 M1 代株系中发现了 1 个雄性 不育株 ,鉴定为细胞核隐性单基因控制的孢子体雄性不育 。 关键词 :玉米 ;γ射线 ;叠氮化钠 ;愈伤组织 ;诱变 ;耐旱 ;雄性不育 D ROUGHT TOL ERANT AND MAL E STERIL E MATERIAL SCREENING FROM γ MAIZE CALL US MUTATED BY RAY AND SODIUM AZIDE FU Fengling 　L I Wanchen 　RON G Tingzhao 　PAN Guangtang 　TAN Dengfeng ( Maize Research Institute , Sichuan Agricultural University , Ya ’an , Sichuan , 625014) 60 γ ( ) Abstract :Maize callus was mutated by Co ray and sodium azide NaN3 , and screened on high osmotic medium with 10 % NaCl . The lines of the regenerated plants were identified for their drought tolerance . The result γ indicated that ray absorption dose of 20 Gy and NaN3 concentration of 1 mmolL were suitable treatment combination to mutate maize callus. From 22 lines of regenerated plants , 5 lines were identified to be more tolerant to drought than control , and one of them had almost the same tolerance with drought tolerant inbred line