生长调节物质对刺槐复叶离体再生的影响.pdf

( ) 核 农 学 报 　2004 ,18 3 :207～211 207 　 Acta Agriculturae N ucleatae Sinica ( ) 文章编号 2004 0320705 生长调节物质对刺槐复叶离体再生的影响 赵 　芳 　李 　云 (北京林业大学生物科学与技术学院 ,北京 　100083) 摘 　要 : 以引进的速生型刺槐的复叶为实验材料 ,进行离体培养 ,研究生长调节物质对刺槐复叶 的小叶和叶轴再生的影响 。以MS 为基本培养基 ,对 IAA 、IBA 、NAA 、2 ,4D 4 种生长素 ,及 BA 、 KT 、ZT 3 种细胞分裂素进行单因子试验 ,对比并筛选出对刺槐小叶和叶轴再生最好的生长素 和细胞分裂素为NAA 、BA 。采用 32 析因设计 ,进行 NAA 、BA 配合试验 ,确定叶轴不定芽分化 的最佳生长调节物质配比为 NAA 05mgL + BA 20mgL ,此时不定芽分化率 7347 % ; 小叶的 不定芽分化的最佳配方为 MS + NAA 10mgL + BA 20mgL ,不定芽分化率为 205 % 。 关键词 :刺槐 ;复叶;再生 ;生长调节物质 EFFECTS OF GROWTH REGULATORS ON REGENERATION OF COMPOUND L EAF in vitr o ON Robinia pseudoacacia ZHAO Fang 　L I Yun ( College of B iological Sciences and B iotechnology , Beij ing Forestry University ,Beij ing 　100083) Abstract :The effects of growth regulators on regeneration of compound leaves of fastgrowing Robinia pseudoacacia were studied . Base on MS medium , four kinds of auxin IAA , IBA ,NAA ,2 ,4D and three kinds of cytokinin BA , KT ,ZT were arranged in monofactoral experiments. NAA and BA were selected to be the best regulators for 2 regeneration of folioles and raches of the compound leaf . Then a 3 factorial design was used to determine the best combination of NAA and BA with different concentration . The results indicated that MS + NAA 05mgL + BA 20mgL was the best medium for adventitious bud regeneration of the raches with the differentiation rate of 7343 % , and to the folioles , it was MS + NAA 10mgL + BA 20mgL with the differentia