四种果皮中橙皮苷含量的测定Determinationof-山东农业大学.PDF

山东农业大学学报(自然科学版),2016,47(3):404-407 VOL.47NO.3 2016 Journal of ShandongAgriculturalUniversity (Natural Science Edition ) doi:10.3969/j.issn.1000-2324.2016.03.016 四种果皮中橙皮苷含量的测定 曹 峰 莱芜市食品药品检验检测中心, 山东 莱芜 271100 摘 要: 为建立一种测定橙皮、橘皮、柚皮及柠檬皮中橙皮苷含量的高效液相色谱法。以Symmertry Shield RP18柱为 分离柱，甲醇–2.5%乙酸溶液 （40:60V:V）为流动相，柱温30 ℃，紫外检测器在波长283nm 条件下检测，一次进样 7min 内完成分析过程。橙皮苷在2.0~100.0mg/L 范围内有良好的线性关系 （R 0.9999）；平均加标回收率为98.9%， –3 2.0×10 g/kg n 6 RSD 0.05% 检出限为 ；精密度实验 （ ）表明，橙皮苷峰面积的相对标准偏差 （ ）为 。该方法简便快 捷，可准确测定四种果皮中橙皮苷的含量。 关键词: 高效液相色谱法; 四种果皮; 橙皮苷 中图法分类号:O657.7 文献标识码:A 文章编号:1000-2324(2016)03-0404-04 DeterminationofHesperidinContentsinFourKindsofPeels CAOFeng LaiwuFood andDrug Inspection Center,Laiwu 271100,China Abstract:In order to develop amethod for determination of hesperidin contents in orange peel, citrus peel, pomelo peel and lemon peel by HPLC. Hesperidin was qualitatively analyzed through a Symmertry Shield RP18 column. The mobile phase was methanol–2.5% acetic acid (40:60 V:V ) and the column temperature was 30 ℃.AUV detector detected at 283 nm and the separation was achieved within 7 min. The standard working curve of hesperidin had a good liner relation in the concentration ranging from 2.0to 100.0mg/L(R 0.9999).The average recovery was 98.9% and detection limit was 2.0×10–3 g/kg. The precision test (n 6) showed that the relative standard deviation (RSD) for the peak areas was 0.05%.This method was simple andrapid and coulddeterminethecontent ofhesperidin in four kinds ofpeels. Keywords:HPLC; four kindsofpeels;hesperidin [1] 橙皮苷 （Hesperidin）是一种双氢黄酮类糖苷 ，为黄酮类化合物中最重要的一种。研究表明， 橙皮苷具有抗氧化、抗过敏、抑菌消炎、抗病毒、抗癌、维持渗透压、降低血压、降低低