棉铃虫中肠氨肽酶APN4与Cry1AcCry2Aa结合能力-应用昆虫学报.PDF

Chinese Journal of Applied Entomology 2015, 52(5): 12291235. DOI: 10.7679/j.issn.20951353.2015.146 棉铃虫中肠氨肽酶APN4 与Cry1Ac、 Cry2Aa 结合能力的比较* 1** 2** 1 1*** 袁向东 葛朝虹 肖玉涛 梁革梅 1. , , 100193 2. , 712100 摘 要 【目的】Bt Bacillus thuringiensis Bt N Aminopeptidase N Bt Bt Bt Bt Bt 【方法】Ligand blot Elisa Helicoverpa armigera APN4Aminopeptidase N4APN4Cry1AcCry2Aa 【结果】APN4 Cry1AcCry2Aa Kd48.59 nmol/L 21.73 nmol/L 【结论】 APN4 Cry1AcCry2Aa 关键词 Bacillus thuringiensis N aminopeptidase N Comparison of the binding affinity of the APN4 receptor in Helicoverpa armigera (Hübner) to the Cry1Ac and Cry2Aa insecticidal proteins 1** 2** 1 1*** YUAN Xiang-Dong GE Zhao-Hong XIAO Yu-Tao LIANG Ge-Mei (1. State Key Laboratory of Insect Pests and Plant Diseases, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 2. College of Plant Protection, Northwest Agriculture and Forestry University, Yangling 712100, China) Abstract [Objectives] Bt (Bacillus thuringiensis) insecticidal proteins have been widely adopted to control agricultural pests because of their high target specificity. The binding of a Bt insecticidal protein to its specific receptor in the insect midgut plays a key role in the insecticidal action process. Aminopeptidase N (APN) is one of the major Bt pro