消岩汤对肺癌A549细胞及肺癌干细胞迁移及侵袭-天津中草药杂志社.PDF

·870 · 中草药 Chinese Traditional and Herbal Drugs 第49 卷 第4 期 2018 年2 月 消岩汤对肺癌 A549 细胞及肺癌干细胞迁移及侵袭的影响 1 1 1 1 1 2 3 1* 李小江 ，张 莹 ，杨佩颖 ，孙彬栩 ，易 丹 ，孙月红 ，姜 珊 ，贾英杰 1. 天津中医药大学第一附属医院 肿瘤科，天津 300193 2. 沧州中西医结合医院 放疗科，河北 沧州 061000 3. 天津中医药大学，天津 300193 摘 要：目的 探讨消岩汤对非小细胞肺癌转移的作用机制。方法 通过划痕实验，观察消岩汤对肺腺癌A549 细胞的迁移 能力的影响；通过侵袭实验，观察消岩汤对肺癌干细胞（SP 细胞）侵袭能力的影响。结果 划痕实验中消岩汤组肺腺癌A549 细胞在24 、48 、72 h 的迁移距离明显短于各时刻对照组细胞（P ＜0.05 ）；侵袭实验中消岩汤干预下的SP 细胞穿出Transwell 小室的细胞数明显少于对照组（P ＜0.05 ）。结论 消岩汤可有效抑制肺腺癌A549 细胞的迁移及SP 细胞的侵袭。 关键词：消岩汤；A549 细胞；肺癌干细胞；迁移；侵袭；非小细胞肺癌转移 中图分类号：R285.5 文献标志码：A 文章编号：0253 - 2670(2018)04 - 0870 - 04 DOI: 10.7501/j.issn.0253-2670.2018.04.019 Effect of Xiaoyan Decoction on migration and invasion of A549 cells and SP cells in lung cancer 1 1 1 1 1 2 3 1 LI Xiao-jiang , ZHANG Ying , YANG Pei-ying , SUN Bin-xu , YI Dan , SUN Yue-hong , JIANG Shan , JIA Ying-jie 1. Department of Oncology, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China 2. Cangzhou Integrated Traditional Chinese and Western Medicine Hospital, Cangzhou 061000, China 3. Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China Abstract: Objective To study the effect of Xiaoyan Decoction on metastasis of non-small cell lung cancer by experimental study. Methods Through the scratch test, the effect of Xiaoyan Decoction on the migration ability of lung adenocarcinoma A549 cells was observed. The transwell migration assay was used to test the role of Xiaoyan Decoction in lung cancer stem cells migration. Results In the scratch test, the migration distance of lung adenocarcinoma A549 cells in 24, 48, and 72 h was significantly shorter than that of the control group at the