小麦NPRlike基因的克隆及赤霉菌诱导下的表达分析.PDFVIP

作物学报 ACTA AGRONOMICA SINICA 2013, 39(10): 1775−1782 / ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2013.01775 小麦NPR1-like 基因的克隆及赤霉菌诱导下的表达分析 杨在东 马 信 吴世文 王宏伟 孙 鑫 冀宪领 李安飞 孔令让* / , 271018 : AtNPR1 , RNA 3 AtNPR1 EST , , 3 cDNA , TaNPR1TaNPR2 TaNPR3, 580607 601 , 3 NPR1-like BTB/POZANK NPR1_like_C , TaNPR1 2 NPR1 , TaNPR1 TaNPR2 TaNPR3 , TaNPR1 NPR1 , TaNPR2 TaNPR3 NPR1 PCR , TaNPR1TaNPR2 TaNPR3 Apogee , Apogee73S2 TaNPR1 TaNPR3 ; TaNPR2 , TaNPR1 TaNPR3 : ; NPR1-like ; ; Cloning of NPR1-like Genes and Their Response to Fusarium graminearum In- fection in Wheat YANG Zai-Dong, MA Xin, WU Shi-Wen, WANG Hong-Wei, SUN Xin, JI Xian-Ling, LI An-Fei, and KONG * Ling-Rang State Key Laboratory of Crop Biology / College of Agronomy, Shandong Agricultural University, Tai’an 271018, China Abstract: NPR1 gene plays a key role for systemic acquired resistance (SAR) and provides broad-spectrum resistance in Arabi- dopsis. Fusarium head blight (FHB), which is caused by Fusarium graminearum Schwabe, is a devastating disease of wheat worldwide. Based on the analysis of the gene expression profiling, we cloned three NPR1-like genes from wheat FHB near-isogenic lines induced by F. graminearum, and they were designated as TaNPR1, TaNPR2, and TaNPR3. The open reading frames of the three genes encod