白藜芦醇对心肌干细胞的分化作用.DOC

Revista de Ciência de Saúde de Macau 澳門醫學雜誌, December 2007, Vol. 7, No. 4 PAGE PAGE 1 修改日期:2007/6/5 ．綜述． 白藜蘆醇對大鼠胚胎 心肌細胞的增殖及分化作用 梁芷穎 李銘源 【摘要】 近年來，白藜蘆醇對心血管系統的保護作用也越來越引起人們的注意，白藜蘆醇通過減少心肌缺血再灌注損傷、舒張血管、抗動脈粥樣硬化、抗低密度脂蛋白氧化等發揮心血管保護作用。除心血管保護作用外，我們的研究揭示白藜蘆醇能誘導大鼠胎心細胞的分化，實驗中，大鼠胚胎心肌細胞株H9c2以不同濃度的白藜蘆醇(30 uM-120 uM)進行培養，在第3天及第5天的培養中，細胞被固定並進行細胞骨架及細核染色，使用瑩光倒置顯微鏡觀察細胞形態；在基因水平上，利用實時合酶鏈鎖反應（real-time PCR）測試肌細胞分化調控基因MyoD1，細胞週期調控因數CDK2等的表達。結果發現白藜蘆醇能使胎心細胞出現分化現象，細胞形態拉長，在實時合酶鏈鎖反應中，白藜蘆醇能上調MyoD1及下調CDK2的表達，表明它通過誘導細胞週期停滯來正向調控細胞分化，提示白藜蘆醇在用於修愎心肌損傷或重建心臟上存在一定的潛能。 【關鍵詞】白藜蘆醇;　　心肌細胞;　　細胞移植 Effect of Resveratrol on Proliferation and Differentiation of Cardiomyoblast Chi Weng LEONG, Simon Ming-Yuen LEE. Floor 3 Block 3, Institute of Chinese Medical Sciences, University of Macao, Macao SAR., China Tel:(+853 E-mail:ma46589@ 【Abstract】 Resveratrol (trans-3,5,4-trihydroxystilbene), a polyphenolic compound found largely in the skins of red grapes, an medicine used to treat diseases of the blood vessels, heart, and liver. Previous report showed that Resveratrol had protective effect against oxidative agent induced. On the other hand, our studies indicated that Reservatrol may place an important role on differentiation of cardiomyoblast. The cardiomyoblast cell line, H9c2, was exposed to 30uM to 120 uM Resveratrol for 3 to 5days. The change of cellular differentiation morphology was observed under microscope. Treatment with Resveratrol up to 4-5 days induced an obvious differentiation of cardiomyblast into myocyte based on morphological examination. Differentiation of H9c2 was further confirmed by measurement of mRNA expression of differentiation marker,myoD1, and cell cycle marker, CDK2. Our results have implication on the role of Reservatrol on cell cycle arrest and differentiation on cardiomyoblast. 【Key Words】Resveratrol;　　Cardiomyoblast;　　Cell Transplantation 白藜蘆醇(Resveratrol)，化學名3,4′,5-三羥基-反-均二苯代乙烯(3,4′,5-trihydroxysitlbene)，是從植物中提取的多酚類天然化學物質。目前，研究表明它具有抗腫瘤、抗心血管疾病、治療突變、抗氧化、抗菌抗炎、保肝、誘導細胞凋亡及