目的意义-第三军医大学学报.DOC

PAGE 1 生物型人工心包的动物试验研究 [基金项目]广东省科技计划项目(2006 彭秀凡 [通信作者]彭秀凡,电话: (020E-mail: p.xiufan@163.com 曾伟生 乔贵宾 蒋仁超 廖明（广州510010，广州军区广州总医院胸处科 [通信作者]彭秀凡,电话: (020E-mail: p.xiufan@163.com （摘要）目的 探讨用生物材料进行心包修复重建的可行性及疗效 方法 对6只中国杂种犬进行右侧开胸，切除直径为8cm类圆形心包，用生物材料人工心包进行修复重建。术后2周、1月、3月、6月、1年、2年分别进行心脏彩超检查，观察有无粘连、积液、破损及增厚钙化。术后1年及2年各解剖2只动物，分别进行大体检查，观察人工心包的内外表面、组织的柔韧性及生物膜性组织有无钙化、增厚及组织学结构；进行组织学检查，观察内膜组织构成及完整性，了解纤维结缔组织层有无血管形成及神经纤维长入。余下2只进行长期观察，以预测人工心包长期置入体内的可能性及转归。 结果 6例动物全部长期存活，心脏彩超动态随访观察未发现人工心包有破损、积液、粘连及钙化增厚等情况发生，大体观察见人工心包浆膜层光滑、无粘连，内膜跨越人工心包与犬正常心包连接处光滑无缺损；组织学检查发现人工心包内膜与正常心包已无缝连接，组织结构相同，在人工心包内见到血管及神经纤维结构。 关键词 生物材料 人工心包 实验研究 Animal experimental study on bioartificial pericardium PENG Xiu-fan, ZENG Wei-sheng, QIAO Gui-bin, et al. Department of Chest Surgery, General Hospital, Guangzhou Military Area, Guangzhou 510010, China Corresponding author: PENG Xiu-fan 【Abstract】 Objective To explore the feasibility and efficacy of pericardial repair and reconstruction with using biomaterial. Methods Through a right extrapleural thoracotomy, eight-diameter circle pericardium was resected in six Chinses mongrel dogs. Subsequently, neoplasty of pericardium was proceeded using biomaterial. The pericardial adherence, effusion, breakage, thickening was evaluated by cardiac color doppler sonography at 6 postoperative time points (2 weeks, 1 month, 3 months, 6 months, 1 year, 2 years after operation). The gross examination was performed at 1 year and 2 years after operation to observe pericardial surface and flexibility and to evaluate calcification and thickening of implanted biomaterial patches. Histological examination was also performed at the same time to observe the construction of pericardial endomembrance and integrity of pericardium. The last 2 animals were used to anticipate the ability of long lasting implanted patches and the relationship between artificial pericardium and post-treatment outcomes. Results All animals survived through all the experiment. No breakag