用于检测生物硫醇的新型萘酰亚胺荧光探针的合成与应用.PDF

第42卷第8期 中国测试 Vol.42 No.8 2016年8月 CHINA MEASUREMENT TEST August，2016 doi院10.11857/j.issn.1674-5124.2016.08.013 用于检测生物硫醇的新型萘酰亚胺荧光 探针的合成与应用 陈 颂袁 王 静袁 侯 鹏袁 刘 磊袁 王 鑫 渊齐齐哈尔医学院药学院袁黑龙江 齐齐哈尔 161006冤 摘 要院该文利用硫醇诱导的2袁4-二硝基苯磺酰基基团的断裂反应生成具有供-吸电能力的荧光团袁成功设计合成 一种基于萘酰亚胺识别硫醇的荧光探针遥 相对于其他氨基酸袁该探针在生理pH渊7.40冤值下袁对于含硫醇的氨基酸具 有高度的选择性和灵敏度遥探针对硫醇具有显著的荧光增强响应袁其荧光强度可以恢复197倍遥此外袁细胞内硫醇的 荧光成像实验袁证明该荧光探针具有潜在检测细胞内生物硫醇的能力遥 关键词院生物硫醇曰合成曰萘酰亚胺曰氨基酸曰断裂反应曰荧光探针 文献标志码院A 文章编号院1674-5124渊2016冤08-0064-04 The synthesis and application of a new type of naphthalimide fluorescent probe for biothiols determination CHEN Song袁 WANG Jing袁 HOU Peng袁 LIU Lei袁 WANG Xin 渊College of Pharmacy袁Qiqihar Medical University袁Qiqihar 161006袁China冤 Abstract: The research used袁 2袁4-dinitrobenzenesulfonyl group cleavage reaction induced by thiols to generate fluorophore with power supply-sucking ability袁 and proposed and synthesized a kind of fluorescence probe that can identify biothiols via naphthalimides. Relative to other amino acids袁 the probe is under physiological pH value 渊7.40冤袁 which has high sensitivity and selectivity on amino acids containing biothiols. The probe has a striking fluorescence enhancement effect on biothiols袁 which can recover fluorescence intensity to 197 times. Besides袁 fluorescence imaging test of biothiols in cells shows that the fluorescence probe has a potential ability of detecting biothiols in cells. Keywords: biothiols曰 synthesis曰naphthalimide曰amino acids曰cleavage reaction曰fluorescence probe 0 引 言 此袁 对这些硫醇化合物的