雌雄栝楼植株内含物比较研究1.PDF

39 2 2008 2 260 中草药 Chinese Traditional and erbal Drugs ¹ * 李　珊, 程　舟 , 李　彦, 刘　晶, 杨晓伶, 朱云国 ( , 200092) : , , HPLC , , , , SOD POD DNA POD MDA, HPLC , HPLC : ; ; : R282. 7: A :0253- 2670( 2008) 02- 0260- 04 Comparison of inclusions in female and male Trichosanthes kirilowii individuals LI Sha , CHENG Zhou, LI Ya , LIU Ji g, YANG Xiao-li g, ZHU Yu -guo ( School of Life Scie ces a d T ech ology , To gji U iversit y, Sha ghai 200092, Chi a) Abstract: ObjectiveT o a alyze the differe t compositio a d co te ts of i clusio i female a d male Tric hosanthes kir ilow ii i dividuals a d to provide some refere ces for the early sex ide tificatio . . Methods Physiological a d biochemical i dices i T kir ilow ii leaves w ere determi ed a d sig ifica t a alysis of the differe ce w as performed. T he differe ce of chemical compositio w as a alyzed usi g HPLC. ResultsT he co te ts of photosy thetic pigme ts a d soluble protei s, the activities of superox- ide dismutase ( SOD) a d peroxidase ( POD) , a d the DNA co ce tratio w ere higher i male i dividuals . tha those i female o es T he differe ce i photosy thetic pigme ts co te t a d POD activity reached a sig ifica t level. The female i dividuals possessed higher co te ts of malo diadehycle ( MDA) , soluble saccharide, a d U V-absorbi g compou ds tha those i male o es, a d the differe ce i UV-absorbi g . , compou ds reached a sig ifica t level HPL