酿酒酵母中提取麦角甾醇的研究-经济学家.PDF

※工艺技术 食品科学 2007, Vol. 28, No. 05 93 薛冬桦，李亚丽，李秀萍，吕君男，马丽娟 (长春工业大学生物工程学院，吉林 长春 130012) ：以酿酒干酵母为原料，在适量甲醇和氢氧化钾溶液中，95℃水浴皂化回流2.5h，用有机溶剂乙醚萃取 麦角甾醇，减压浓缩富集。4～－ 7℃缓慢降温结晶，获得麦角甾醇粗品，产率为1.11%。将麦角甾醇粗品溶解 于乙醚溶剂中，重结晶得到纯度较高的麦角甾醇晶体，平均产率为0.69%，提取率可达62.58%。采用电子扫描显 微镜对恒温结晶和降温结晶得到的晶体进行晶貌观察，利用红外光谱法进行麦角甾醇的结构表征。 ：麦角甾醇；酵母；提取；结晶 Study on Extraction Process of Ergosterol from S.cervisiae Dry Yeast .. XUE Dong-hua，LI Ya-li，LI Xiu-ping，LU Jun-nan，MA Li-juan (School of Biological Engineering, Changchun University of Technology, Changchun 130012,China) S.cervisiae as raw material, dissolve it in the solution of 20% potassium hydroxide, saponify and reflux Abstract ：Dry yeast －7 ℃ to get ergosterol it for 2.5 h at 95 ℃, extract with diethyl ether, concentrate it and drop gradually it in temperature from 4 ℃ to crystal. The next step is to dissolve the crude product in the diethyl ether and to recrystalliz with gradual temperature lowering, so the crystal of the ergosterol is obtained. The yield of the ergosterol crystal is 0.69% (relative to the dry yeast), and the extract rate can achieve 62.58%. Scanning electron microscope (SEM) is employed to observe the morphology of the ergosterol and the infrared furrier spectrometer is used to record the infrared spectrum of the ergosterol. Key words：ergosterol；yeast；extract；crystal 中图分类号：Q548 文献标识码：A 文章编号：1002-6630(2007)05-093-04 麦角甾醇，是脂溶性 V D 2 的前体，当受到紫外线 的提取率，以适应工业化生产。 照射时可转化成VD2 [1]，而且也是甾醇类激素药物的原 料，在食品、医药和饲料工业应用广泛[ 2 ] 。目前，我