白果过敏蛋白及其过敏原性研究.PDFVIP

16 2011, Vol. 32, No. 07 食品科学 ※基础研究 1 1 , 2 1 李莹莹 ，吴彩娥 * ，杨剑婷 ，范龚健 (1.南京林业大学森林资源与环境学院，江苏 南京 210037 ；2.安徽科技学院食品药品学院，安徽 凤阳 233100) 对白果的主要过敏蛋白进行鉴定，并对其过敏原性进行分析。以 SDS和 Western-Blotting 分析鉴定 白果蛋白提取液中的蛋白质组分和过敏蛋白，通过间接ELISA 法检测过敏小鼠血清中 sIgE 的效价。白果的蛋白质 条带主要有 13 条，其中以 21kD 和 32kD 含量最多。Western-Blotting 分析结果表明，白果蛋白中存在 3 条过敏原蛋 白，分子质量分别为21 、32 、36kD 。当酶标二抗稀释度为 1:1000，白果蛋白包被质量浓度为50 μg/mL ，间接 ELISA 测得过敏小鼠血清中 sIgE 的效价为 1:6400。对白果蛋白进行分析鉴定，明确了白果中存在的过敏原蛋白。 白果；过敏蛋白；SDS；Western-Blotting ；间接ELISA Identification and Allergenicity of Allergic Proteins in Ginkgo Seeds 1 1, 2 1 LI Ying-ying ，WU Cai-e *， YANG Jian-ting ，FAN Gong-jian (1.College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China ； 2.College of Food and Drug, Anhui Science and Technology University, Fengyang 233100, China) Abstract：SDSand Western-Blotting were used to identify the protein composition and allergic proteins of ginkgo seed extract, respectively. A total of 13 bands were observed in the SDSpattern, and the fractions of 21 kD and 32 kD exhibited the highest content. Western-Blotting analysis indicated that ginkgo seeds had 3 allergic proteins, whose molecular weights were 21, 32 kD and 36 kD, respectively. When the enzyme secondary antibody concentration was 1:1000 and the ginkgo protein coating concentration was 50 μg/mL, the serum IgE titer of mice allergic to ginkgo seeds was detected to be 1:6400 by indirect ELISA. Key words ：ginkgo seed ；allergic protein ；SDS；Western-Blotting ；indirect ELISA 中图分类号：Q816 文献标识码：A