合成生物学与基因回路.ppt

Information vs. Processing Just as in a computer, data bits and processing bits are made from the same material, 0 or 1, or A, T, C, G, or U in biology Nature as a Computer Biological systems like DNA and RNA especially appear to be more than networks of information. RNA itself can be seen as a molecular decision network E-Cell E-Cell System is an object-oriented software suite for modeling, simulation, and analysis of large scale complex systems such as biological cells. Version 3 allows many components driven by multiple algorithms with different timescales to coexist Computer Modeling Metabolic Pathways BioCyc – collection of organism specific metabolic pathway databases cellML is an XML based format for exchanging biological data from genes to proteins to metabolism Digital Cells MeetSynthetic Biology Model the circuit Validate the circuit Tinker with the circuit Then… Alter the gene to build a new protein SNPs will give you a ‘first approach’ See if the new protein is ‘well tolerated’ Gene Therapy Gene therapy using an Adenovirus vector. A new gene is inserted into an adenovirus vector, which is used to introduce the modified DNA into a human cell. If the treatment is successful, the new gene will make a functional protein. /wiki/Gene_therapy DNA Vaccines The ultimate method to train the immune system against a multitude of threats Inject a known sequence of DNA Trick the cell into expressing it, then seeing it as an antigen to ward against. Used to fight cancer. Animal Model Systems Mice make perfect models – as they are: Cheap (reasonably) Fast / easy growing Very ‘inbred’ Mouse DNA arrays and the mouse genome are fairly well known, characterized Stem Cell Technology Once you have an ‘altered genome’ ready to test beyond a simple one cell environment, you leverage the ability of stem cells to ‘mass produce’ your synthetic biology solution Cell as a Nanosystem Bilayer outer lipid membrane Energy apparatus Diffuse metabolome Proteome with signaling networ