滋肾平颤颗粒对异动症大鼠行为学及纹状体多巴胺D受体活性与基因表达影响.pdfVIP

Traditional Chinese Medicine 中医学, 2017, 6(2), 118-126 Published Online May 2017 in Hans. /journal/tcm /10.12677/tcm.2017.62020 Effect of Zishenpingchan Granule on Neurobehavioral Manifestations and the Activity and Gene Expression of Striatal Dopamine D1 Receptors of Rats with Levodopa-Induced Dyskinesias 1 2 1 1 1 Qing Ye , Dongwen Gao , Jie Zhou , Xiaolei Yuan , Canxing Yuan 1 Department of Neurology, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 2 Department of Ultrasonography, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai th rd th Received: May 11 , 2017; accepted: May 23 , 2017; published: May 26 , 2017 Abstract Objective: To observe the effects of Traditional Chinese Medicine on neurobehavioral manifesta- tions and the activity and mRNA expression of striatal dopamine D1 receptors of rats with levo- dopa-induced dyskinesias (LID). Methods: The rat model of Parkinson’s disease (PD) was estab- lished by 6-OHDA; then, the model of LID in rat was produced by injecting levodopa (LD) and ben- serazide for 4 weeks. The rats were divided into normal control group, LID model group and TCM intervention group. After 4 weeks of treatment, the effect of TCM on abnormal involuntary move- ment (AIM) score of rats with LID, peak dose rotation and efficacy time of levodopa (LD) were ob- served. The maximum binding capacity (Bmax) and equilibrium dissociation constant (KD) of striatal dopamine D1 receptors of rats were determined. The mRNA expression of