低背景高分辨率PAGE简易银染法-遗传.PDF

HEREDITAS (Beijing) 2009 6 , 31(6): 668―673 ISSN 0253-9772 技术与方法 DOI: 10.3724/SP.J.1005.2009.00668 低背景、高分辨率PAGE 简易银染法 高东, 杜飞, 朱有勇 , , , 650201 聚丙烯酰胺凝胶电泳银染一直存在耗时长、步骤繁琐等缺陷, 是其批量应用的瓶颈。文章报道了一种低 背景、高分辨率的PAGE 简易银染方法, 该法在降低NaOH 浓度和批量显带方面进行了有益的探索, 建立了节 省时间、节约试材, 对批量显带尤为实用的简易银染法。 聚丙烯酰胺凝胶; 银染; 显影 Low-background and high-resolution contracted silver-stained method in polyacrylamide gels electrophoresis GAO Dong, DU Fei, ZHU You-Yong The National Center for Agricultural Biodiversity, Ministry of Education Key Laboratory of Agricultural Biodiversity for Plant Disease Management , Key laboratory of Plant Pathology, Yunnan Agricultural University, Kunming 650201, China Abstract: The disadvantage of time-consuming and fussy steps of conventional silver-stained method for polyacrylamide gels is evident, and has become the choke point in its application. In this paper, a low-background and high-resolution con- tracted silver-stained method for polyacrylamide gels was established. Compared with the conventional banding method, this contracted method has fewer steps and needs fewer reagents, in particular, lower NaOH concentration. It is rapid and economic, especially for mass silver-staining. Keywords: PAGE (polyacrylamide gel electrophoresis); silver staining; banding (Polyacrylamide gel electro- 1 phoresis, PAGE) 1959 , 1.1 [1~4] [5, 6] , [7] DNA; SSR , Bassam[8]PAGE, RM335 RM225, 1 , 1.2 [9~15] 1.2.1 PCR 反应体系和程序 PAGE,