均匀试验设计优选青黛提取工艺-中国现代应用药学.PDF

均匀试验设计优选青黛提取工艺 段晓颖，高卫芳，张辉，闫艳仓，吴彩丽(河南中医学院第一附属医院，国家中医药管理局中药制剂三级实验室，郑州 450000) 摘要：目的 通过均匀试验优选青黛中靛玉红的最佳提取工艺。方法 以靛玉红提取率为指标，对青黛醇提过程中加醇 量、乙醇浓度、提取时间及提取次数 4 个因素进行筛选。结果 影响靛玉红提取率的各因素作用大小顺序为：乙醇浓度 ＞溶剂量＞提取时间＞提取次数，其中乙醇浓度，提取次数，溶剂量与靛玉红提取率呈正相关。结论 青黛最佳提取工 艺为加90%乙醇9 倍量，提取2 次，每次0.5 h 。 关键词：青黛；靛玉红；均匀试验；提取工艺 基金项目：河南省科技攻关项目(072102330035) 作者简介：段晓颖，女，硕士，主任药师，硕导 Tel: (0371E-mail: dxy137@ ·326 · Chin JMAP, 2012 April, Vol.29 No.4 中国现代应用药学2012 年 4 月第 29 卷第 4 期 中图分类号：R284.2 文献标志码：B 文章编号：1007-7693(2012)04-0326-04 Optimal Extraction Technology of Indigo Naturalis by Uniform Design DUAN Xiaoying, GAO Weifang, ZHANG Hui, YAN Yancang, WU Caili(The First Affiliated Hospital of Henan University of Trational Chinese Medicine, the Third Grade Laboratory of State Administration of Trational Chinese Medicine, Zhengzhou 450000, China) ABSTRACT: OBJECTIVE To optimize extraction process of Indigo Naturalis by uniform design. METHODS The process was studied by uniform design method with the extraction ratio of indirubin as the index, and four factors were chosen, including ethanol volume, ethanol concentration, extraction time and the extraction times. RESULTS The factors influenced the indirubin extraction was ethanol concentration ＞ethanol volume ＞extraction time ＞extraction times. Extraction time, ethanol volume and ethanol concentration were positively correlated with the extraction efficiency. CONCLUSION The results indicate that the optimum extraction technology is as follows: 10 times volume of 90% ethanol, 0.5 h reflux and extraction for 2 times. KEY WORDS: Indigo Naturalis; indirubin; uniform experiment; extraction process 青黛为爵床科植物马蓝 Baphicacanthus cusia 2 方法及结果 (Nees) Bremek.，蓼科植物蓼蓝Polygonum tinctorium 2.1 均匀实验设计 Ait.或十字花科植物菘蓝Isatis indigotica Fort. 的叶 靛玉红为青黛的主要有效成分，因此以提取