基于表面等离子体共振技术检测食品中的黄曲霉毒素B1-食品科学与工程专业论文.docxVIP

北京化工大学硕士学位论文 北京化工大学硕士学位论文 于CM5芯片上，捕获生物素修饰的AFBl核酸适配体；优化AFBl 的进样和解离时间，建立AFBl标准品的工作曲线。AFBl的浓度在 0．19～200 ng·mL～，浓度与相应的响应信号成四因素关系；AFBl的浓 度在0．19～50 ng·mL一，浓度与相应的响应信号成线性关系。 3、通过优化酱油和醋样品基质中AFBl的提取和纯化方法，用已 建立的SPR与适配体结合的方法完成酱油和醋样品中AFBl的检测。 当醋中AFBl浓度为2、10、20 ng·g。1(相对于醋的质量)，该方法的 平均回收率在94．1～125．9％范围内。 4、建立液质联用方法检测AFBl，得到的结果与SPR方法做对比。 该方法的线性范围为1～200 ng·mL～，检出限为0．5 ng-mL一。 5、建立液相方法检测AFBl，得到的结果和SPR方法做对比。该 方法的线性范围为1．56～200ng·mL一，检出限为0．78 ng·mL～。 关键词：AFBl检测，SPR，适配体，醋检测，HPLC 万方数据 ABSTRACTDETERMINATION ABSTRACT DETERMINATION oF AFLATOⅪN Bl INⅥNEGAR BY SURFACE PLASMON RESPONSE BIOSENSoR WITH APTAMER ABSTRACT Aflatoxins are produced by Aspergillus务Qvus which may contaminate food and agricultural products．The aflatoxins Can be divided into six categories：aflatoxin B 1(AFB 1)，aflatoxin B2(AFB2)，aflatoxin G 1(AFG 1)，aflatoxin G2(AFG2)，aflatoxin M 1(AFM 1)and aflatoxin M2(AFM2)．Aflatoxin B 1(AFB 1)is acutely toxic and the most carcinogenic amongst all mycotoxins．Because mycotoxins pose health risks for humans and animals，their levels are monitored throughout many countries and organizations．For example，the Chinese Standard has set maximum levels of 5．0峪’kffl for aflatoxin B1 in the seasoning． Therefore，it is very important to establish sensitive，selective and simple methods for the determination of AFB 1 in food and agricultural products． Surface plasmon resonance(SPR)is an optical phenomenon which occurs at the interface between thin conducting films and media of different refractive indexes． Aptamers are single-s仃anded oligonucleotides，and they can specifically bind to a target molecule．In this research，we reported a sensitivity and rapid method for detection of aflatoxin B 1 by SPR biosensor with aptamer technology． III 万方数据 北京化工大学硕士学位论文1．Based 北京化工大学硕士学位论文 1．Based on the research of literature，the sequence of AFB 1一aptamer in our study：bio-5-GTTGGGCACGTGTTGTCTCTCTGTGTCTCGTG CCCTTCGCTAGGCCCACA一3 7．To confirm the binding status and intensity of AFB 1 to aptamer,we perform ITC exper