家蚕β-呋喃果糖苷酶基因BmSuc1的CRISPR敲除载体-应用昆虫学报.PDF

Chinese Journal of Applied Entomology 2018, 55(2): 208216. DOI: 10.7679/j.issn.20951353.2018.029 家蚕β-呋喃果糖苷酶基因BmSuc1 的 CRISPR 敲除载体的构建与导入* 1** 1, 2** 1 1, 2 1 1 朱文恺 甘 泉 贺 伟 张新伟 周 跃 吴梦雪 孙同同1 1 1, 2*** 江 松 孟 艳 (1. 230036 2. 230036 摘 要 【目的】CRISPR/Cas9 BmSuc1 Bombyx mori β-β-fructofuranosidase β-FFase β-FFase β-FFase BmSUC1 BmSuc1 CRISPR/Cas9 sgRNA CRISPR BmSuc1 【方法】BmSuc1 ORF 2 sgRNACRISPR Sal I Nhe I G0 G1 【结果】 PCR CRISPR DsRed2 G1 【结论】 sgRNA CRISPR BmSuc1 - 关键词 CRISPR/Cas9sgRNAβ- Construction of a CRISPR vector to edit the β-fructofuranosidase gene BmSuc1 and its incorporation in the silkworm Bombyx mori ZHU Wen-Kai1** GAN Quan 1, 2** HE Wei 1 ZHANG Xin-Wei 1, 2 ZHOU Yue 1 WU Meng-Xue1 SUN Tong-Tong 1 JIANG Song 1 MENG Yan 1, 2*** (1.School of Life Sciences, Anhui Agricultural University, Hefei 230036, China; 2.Anhui International Joint Research and Development Center of Sericulture Resources Utilization, Hefei 230036, China) Abstract [Objectives] CRISPR/Cas9 is a novel genome-editing tool that has recently had broad application in many fields of research. CRISPR/Cas9 has the advantages of simplicity, efficiency and high specificity. BmSuc1 encodes β-fructofuranosidase (β-FFase, a fructosyl-hydrolytic sucrase) in the silkworm Bombyx mori (Lepidoptera). BmSuc1 is also the first β-FFase gene to have been cloned and identified in an animal. The physiological function of BmSUC1 in the silkworm is probably related to defense against mulberry alkaloids. However, fu