竹材木质素高效降解菌的分离筛选及鉴定-中南林业科技大学学报.PDF

第34 卷 第8 期 中 南 林 业 科 技 大 学 学 报 Vol. 34 No. 8 2014 年8 月 Journal of Central South University of Forestry Technology Aug. 2014 竹材木质素高效降解菌的分离筛选及鉴定 刘 剑，刘君昂，周国英，李 河，杨 菁 （中南林业科技大学 经济林培育与保护省部共建教育部重点实验室，湖南长沙4 10004 ） 摘 要：采用愈创木酚变色、苯胺蓝平板退色和酶活性测定筛选出高效降解竹材木质素的菌株。结果表明：通 过腐烂的竹材等 34 份样品分离纯化出 139 株真菌 ; 通过 0.04% 愈创木酚和 0.1‰苯胺蓝平板初筛，从分离纯化 的 139 株真菌中筛选出具有产木质素降解酶活性的 7 株降解菌；7 株降解菌经液体产酶培养基复筛得到 2 株高 效产降解木质素酶活的菌株：培养 7 d，漆酶、木质素过氧化物酶和锰过氧化物酶活性分别达 72.558、23.769、 55.044 和 34.611、38.781、82.646 U/mL ；2 株降解菌 FG-35 和 FG-98 分别鉴定为革耳菌 Panus lecomtei 和烟管菌 Bjerkandera adustus 。 关键词：竹材；木质素；降解菌；分离筛选；鉴定 中图分类号：S795；S718.8 文献标志码：A 文章编号：1673-923X(2014)08-0048-05 Isolation, screening and identification of high effective bamboo-lignin degradation fungus LIU Jian, LIU Jun-ang, ZHOU Guo-ying, LI He, YANG Jing (Key Lab. of Cultivation and Protection for Non-Wood Forest Trees Co-constructed by Provinces and State Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, Hunan, China) Abstract: Fungal strains with highly lignin-degrading enzymes activity were screened by colorful reactions of guaiacol-PDA plates, aniline blue-plates and determination of enzymes activity. The results indicate that the 139 fungal strains of fungi were isolated and purified from 34 decayed bamboo samples; And the 7 fungal strains with highly enzymes activity were finally obtained from 139 fungal strains by using guaiacol-PDA plates (containing 0.04% guaiacol) and aniline blue-plates; Then the 7 fungal strains were taken into the liquid medium for determining their ’s enzymes activity; Finally, t