水稻抗纹枯病突变体的离体筛选敖世恩-华南农业大学学报.PDF

水稻抗纹枯病突变体的离体筛选 敖世恩，杨 媚，周而勋，唐倩菲，潘汝谦 (华南农业大学植物病理学系，广东 广州 510642) 摘要：以水稻成熟胚培养产生的愈伤组织和悬浮细胞为筛选材料，以广东省水稻纹枯病菌RhizoctoniasolaniKuhn 强致病力菌株GD-11和GD-118产生的粗毒素为选择压力，从水稻品种的选择、粗毒素致病力的鉴定、突变体的筛 选方法、突变体的再生以及突变体再生植株对粗毒素的抗病性鉴定等方面展开研究，建立了水稻抗纹枯病突变体 的筛选体系并获得了抗病突变体．筛选体系为：两步筛选法，即以粤香占的愈伤组织和悬浮细胞为筛选材料，以ψ (粗毒素)为3．5％和4．0％分别作为第1、2步的处理浓度，每一步处理的时间为20d；筛选出的突变体再生苗的病 情指数为37．55，而原始型再生苗的病情指数为87．50，抗病效果达57．08％． 关键词：水稻纹枯病；水稻纹枯病菌；粗毒素；抗病突变体 中 图分 类 号 ：S435．111．4 文 献 标 识 码 ：A 文 章编 号 ： 1001-411X(2006)01-0047-04 ScreeningofRiceSomaticMutantsResistanttoRiceSheathBlightinvitro AO Shi-en, YANG Mei, ZHOU Er-xun,TANG Qian-fei,PAN Ru-qian (DepartmentofPlantPathology,SouthChinaAgric.Univ.,Guangzhou510642,China) Abstract:Ricesomaticmutantsresistanttoricesheathbhghtwerescreenedinvitrowithcalliandsuspen- sioncellsderivedfromricematureembryocultureandbyusingpathogencrudetoxinextractedfromthe culturefiltratesofstrongvirulentisolatesGD-11andGD-118ofricesheathblightpathogenRhizoctonia solaniKtihncolletedfromGuangdongProvince.Inthisthesis,studieswerecarriedoutonthechoiceof ricecultivars,virulenceidentificationofcrudetoxinondetachedriceleaves,mutantscreeningmethods, regenerationofmutantplant,andresistanceidentificationofregenerativeplanttocrudetoxin.Thus,the systemofinvitroscreeningsomaticmutantsforricesheathblightresistancewasestablished,andthemu- tantsresistanttoricesheathblightweregainedthroughthisstudy.Thescreeningsystemistwo-stepmeth- od,i.e.resistantsomaticmutantswerescreenedwithcalliandsuspensioncellsofricecuhivarYuexian- gzhanandbyusingpathogencrudetoxinconcentrationof3.5%and4.0%forthefirst-andsecond-step respectively,thetreatmenttimeforeachstepwas20days;Thediseaseindexofregenerativeplantofmu- tantwas37.55,whilethatofregenerativeplantoforiginalricewas87.50,theeffectofresistancewas 57.08%. Key words:rice sheath blight;Rhizoctonia solani;crude toxin;resistantmutant