胎生蜥蜴消化道4种内分泌细胞的免疫组织化学研究-Lacertade.PDF

中国农学通报 2012,28(23):56-60 Chinese Agricultural Science Bulletin 胎生蜥蜴消化道4种内分泌细胞的免疫组织化学研究 吴 昊，李淑兰，刘志涛，刘 鹏，赵文阁 （哈尔滨师范大学生命科学与技术学院，哈尔滨150025） 摘 要：为了探索胎生蜥蜴消化道内分泌细胞的形态与分布规律。应用胃泌素(Gas)、胰高血糖素(Glu)、 胰多肽(PP)和P-物质(SP) 4 种特异性抗血清，对胎生蜥蜴(Lacerta vivip ara) 消化道内分泌细胞进行了免 疫组织化学定位研究和形态学观察。用免疫组织化学ABC 法(avidin-biotin compex method) 以揭示其消 化道内分泌细胞的分布规律及特点。结果表明：Gas 细胞分布在贲门、幽门和小肠，其中以十二指肠处 分布密度最高，贲门次之。Glu 细胞主要分布在幽门和小肠前段，小肠后段偶见，并且幽门处的分布密 度明显高于其他部位。在幽门、十二指肠和回肠都检测到了PP 细胞，并且在十二指肠分布最多。仅在 幽门部检测到了少量的SP 细胞。4 种内分泌细胞以圆形和锥体形为主，它们广泛分布于上皮细胞之间、 腺泡上皮细胞之间及上皮细胞基部。上述内分泌细胞的分布特点可能与其食性、食物组成和生活环境 有关。 关键词：胎生蜥蜴；消化道；内分泌细胞；免疫组织化学 中图分类号：Q955 文献标志码：A 论文编号：2012-0585 ImmunohistochemicalStudyoftheFourKindsofEndocrineCells intheDigestiveTractofLacertavivipara Wu Hao, Li Shulan, Liu Zhitao, Liu Peng, Zhao Wenge (CollegeofLifeScienceandTechnology,HarbinNormalUniversity, Harbin 150025) Abstract:To clarify the morphological features and region distribution of the endocrine cells in the digestive tract of the Lacerta vivipara. The localization and morphology of endocrine cells in the digestive tract of Lacertavivipara were studied with Gastrin (Gas), Glucagon (Glu), Pancreatic Polypeptide (PP) and substance P-(SP) four kinds of specific antisera. Immunohistochemical ABC method (avidin-biotin complex method) was used to reveal the law and characters of endocrine cells in the digestive tract. The results showed that Gas cells located in the cardia, pylorus and small intestine, with the highest density in the duodenum, followed by the cardia. Glu cells were mainly in the pylorus and the anterior small intestine, the back of the small intestine occasionally, and the pylorus