胶原白藜芦醇支架的构建及其在软骨修复中的应用-材料学专业论文.docxVIP

ABSTRACT In this work, the water-solubility of resveratrol (RSV) was first synthesised by coupling with polyacrylic acid (PAA, Mn= 1000) via 1, 1-carbonyldiimidazole (CDI). The resulted PAA-RSV showed much better water solubility than RSV. The PAA-RSV still kept the ability to capture DPPH free radicals produced by H2O2. PAA-RSV and collagen were then cross-linked by EDC/NHS to construct collagen/RSV scaffold. The compressive strength of collagen/RSV scaffold was about 1.1 MPa. The cell compatibility of hydrogel was evaluated by MTT method. The results showed that the hydrogel can promote the proliferation of chondrocytes and BMSCs. Comparing with collagen scaffold, the collagen/RSV scaffold showed better compatibility. The chondrocytes can grow better on the collagen/RSV scaffolds and secrete more glycosaminoglycans. The BMSCs and chondrocytes onto the collagen/RSV scaffold exhibited a better viability than those on collagen hydrogel after exposed to H2O2. The collagen and collagen/RSV scaffolds were transgrafted into the articular cartilage defects of rabbit, respectively. After 12 weeks, the recovered tissue was evaluated. The collagen/RSV scaffoldshave demonstrated better recovery than collagen scaffold. It is anticipated that this collagen/RSV scaffold is promising to be applicable in in treatment of arthritis. KEY WORDS: resveratrol; anti-inflammation; BMSCs; chondrocyte; tissue engineering 目 录 HYPERLINK \l _bookmark0 第一章 绪论1 HYPERLINK \l _bookmark1 1.1 关节炎1 HYPERLINK \l _bookmark2 1.2 组织工程2 HYPERLINK \l _bookmark3 1.2.1 软骨组织工程3 HYPERLINK \l _bookmark4 1.2.2 软骨组织工程种子细胞3 HYPERLINK \l _bookmark5 1.2.3 组织工程治疗关节炎的材料4 HYPERLINK \l _bookmark6 1.2.4 软骨组织工程中的生物活性物质7 HYPERLINK \l _bookmark7 1.2.5 关节炎软骨修复-抗炎症材料 8 HYPERLINK \l _bookmark8 1.3 胶原的简介8 HYPERLINK \l _bookmark9 1.3.1 胶原的结构8 HYPERLINK \l _bookmark10 1.3.2 胶原的性质9 HYPERLINK \l _bookmark11 1.3.4 胶原生物材料10 HYPERLINK \l _bookmark12 1.3.5 胶原基生物材料的研究现状 11 HYPERLINK \l _bookmark13 1.4 白藜芦醇的简介