金沙绢毛菊的化学成分研究-中药学中药化学专业论文.docxVIP

金沙绢毛菊的化学成分研究 金沙绢毛菊的化学成分研究 PAGE PAGE 2 ABSTRACT Soroseris gillii (S. Moore) Stebb. is a species of genus of Soroseris Stebbins, and it is a perennial herb, which mainly distribute in Sichuan Province and Qinghai Province of China. It has long been used as a traditional Tibetan medicine for the treatment of food poisoning and rheumatic disease. It also can clear away heat and toxic material. Only a few studies on the chemical constituents and pharmacology of Soroseris Stebbins have been made. A lot of flavones, sterols, monoterpenes and sesquiterpenes have been isolated and evaluated from Soroseris erysimoides (Hand.-Mazz) Shih. The chemical constituents of the 95％ ethanolic extract of Soroseris gillii (S. Moore) Stebb. were studied and eleven compounds were isolated by means of crystallization, recrystallization, chromatography on silica gel column and polyamide column ect. Their structures were identified by spectroscopic methods such as MS, 1H-NMR, 13C-NMR，DEPT techniques and by comparison of their physical and spectral data with those reported in the literature. The eight chemical constituents were elucidated as: luteolin (1), luteolin-7-O-β-D- glucoside (2), caffeic acid (3), lupeol acetate (4), ursolic acid (5), stigmasterol (6), β-sitosterol (7), daucosterol (8). Meanwhile, the microscopic identification, the thin layer chromatography identification, and determination of total flavonoids and luteolin-7-O-β-D-glucoside and luteolin content of the quality of Soroseris gillii (S. Moore) Stebb. were studied. In this study, it has been found that Soroseris gillii (S. Moore) Stebb. is rich in flavonoids. The study will provide theoretical evidences for its rationality of the folk medication and utilizing plant resources. KEY WORDS：Soroseris gillii (S. Moore) Stebb.; chemical constituents; structure identification; HPLC; Determination of content 符号说明 符号名 ESI-MS 英文名称 Electrospray ionization mass spectrum 中文名称 电喷雾质谱 EI-MS 1H-NMR 13C-NMR Electron impact ionization mas