中华鲟半胱氨酸蛋白酶抑制剂C基因的原核表达-水产学报.PDFVIP

27 3 Vol. 27, No. 3 2003 6 JOURNAL OF FISHERIES OF CHINA June, 2003 : 1000- 0615( 2003) 03- 0239- 06 C 1, 2 1 1 1 1 1 马冬梅 , 白俊杰 , 劳海华 , 简 清 , 叶 星 , 罗建仁 ( 1. , 510380; 2. , 520425) : ( cystatin) , (Acipenser sinensis) cystatin C cDNA pBV220, cystatin C SDSPAGE , 12.4 kD , 25% 85% , cystatin C cystatin C : ; ; ; : S917.4 :A Expression of Acip enser sinensis cystatin C in prokaryote 1,2 1 1 1 1 1 MA Dongmei , BAI Junjie , LAO Haihua , JIAN Qing , YE Xing , LUO Jianren ( 1.KeyLaboratory of Tropical Subtrop ical Fish Breeding Cultivation Certfiicated by the inistry of Agriculture. Pearl River Fishery ResearchInstitute , ChineseAcademy of Fishery Sciences, Guangzhou 510380, China 2. Zhanjiang Ocean University, Zhanj iang 520425, China) Abstract : In order to explore the functions of fish cystatin and the potential values in the fish disease prevention and cure, as well as seafood process industry, polymerase chain reaction ( PCR) technique was used to modify the cDNA of Chinese sturgeon ( Acipenser sinensis) cystatin mature peptide, The cDNA was su cloned into expression vector pBV220, then a cystatin recom inant expression acteria was constructed. After temperature inducement and SDSPAGE analysis, the recom inant expression acteria produced a special protein a out 12. 4 kD in molecular weight. The proportion of recom inant protein in total acterial protein was a out 25%. The purity of the recom inant cystatin, which was washed, dissolved, dialysed and renatured, was a out 85%. The inhi itory activity of recom inant cystatin could e measured y inhi iting the prote