局麻药长时间阻滞外周神经对神经源性疼痛发生过程中背根神经节内GAP-43表达的影响-麻醉学专业论文.docxVIP

汕头大 汕头大学医学院硕士学位论文 PAGE PAGE 10 Abstract Objective By using immunohistochemistry method, we observed the effect of continuous peripheral nerve block by local anesthetics on growth associated protein-43(GAP-43) expression during the development of neuropathic pain. Methods Thirty-five SD rats were randomly divided into four groups: control group; simple sciatic nerve section(axotomy) group; peripheral nerve block before section group; peripheral nerve block after section group. Each group was subdivided equally into 2 groups according to the different postoperative interval: 3 and 7 days. The right sciatic nerve were exposed and transected at 1cm distal to sciatic notch. The peripheral nerve block groups were injected with local anesthetics 1 hour before or 4 hours after operation respectively. At various interval after surgery, the dorsal root ganglions to the transected sciatic nerve were harvested. Immunohisto- chemistry and image analysis were used to evaluate the expression of GAP-43. Results At the third and seventh day after surgery, the GAP-43 expression in the simple sciatic nerve section group was higher than that in control group; while both in peripheral nerve block before and after surgery groups the expression of GAP-43 had no different from that in control group. The study suggests that continuous peripheral nerve block 1 hour before or 4 hours after surgery can suppress the high expression of GAP-43 during the development of neuropathic pain. Conclusions 1. GAP-43, which regulates the regeneration and recombination of axons after nerve injury, is the molecule marker of neuronal plasticity and plays an important role in the induction of long-term potentiation(LTP). We found the high expression of GAP-43 in the mouse sciatic nerve transection model of neuropathic pain, which suggests that the GAP-43 involve in the development of neuropathic pain. Continuous blockage of peripheral nerve during the development of neuropathic pain, suppressing the trans