阿萨希毛孢子菌氟康唑体外诱导耐药的方法优化.docVIP

阿萨希毛孢子菌氟康唑体外诱导耐药的方法优化 张德全1，2 ，夏志宽2 ，田艳丽2 ，廖勇2 ，吕雪莲2 ，陈卫2，王聪敏2，杨蓉娅2(400038 重庆，第三军医大学1；100700 北京，北京军区总医院全军皮肤损伤修复研究所2) [摘要] 目的 观察氟康唑联合甲强龙体外诱导阿萨希毛孢子菌(Trichosporonasahii，T.asahii)获得氟康唑体外诱导耐药株的成功率及稳定性，为T. asahii唑类耐药机制研究奠定基础。方法 对16株T. asahii进行氟康唑的真菌体外药敏试验，分别通过氟康唑、氟康唑联合甲强龙和单纯甲强龙对其进行体外诱导耐药，并与空白对照组进行比较。所有诱导获得的耐药菌株进行30次无药传代，每株菌在每次传代后均重新进行最小抑菌浓度（MIC）检测。结果 空白对照组及单纯甲强龙组各菌株MIC值无明显变化；单纯氟康唑诱导获得耐药株12株（75%），氟康唑联合甲强龙诱导获得耐药株16株（100%）；两组经30代传代后，单纯氟康唑组耐药株6株（37.5%），氟康唑联合甲强龙诱导获得耐药株9株（56.25%）。结论 甲强龙可以提高氟康唑诱导T.asahii 耐药株的成功率和稳定性，氟康唑联合甲强龙优于单纯氟康唑，更适用于T.asahii氟康唑体外耐药株的诱导。 [关键词]阿萨希毛孢子菌；氟康唑；耐药性；最小抑菌浓度 [中图法分类号] [文献标志码]A Optimization on induction offluconazole resistance in vitro inTrichosporonasahii ZHANG De-quan1，2，XIA Zhi-kuan2，TIAN Yan-li2，LIAO Yong2，LU Xue-lian2，CHEN Wei2，WANG Cong-min2，YANG Rong-ya2 (1. 400038，Chongqing，Third Military Medical University;2. 100700，Beijing，The Military Institute of Injury and Reparation，General Hospital of Beijing Military Command of PLA)[基金项目] 国家自然科学基金（ [基金项目] 国家自然科学基金 [通信作者] 杨蓉娅，电话（010E-mail: yangrya@ [Abstract]Objective To compare thesuccessful rate and stability of fluconazole induction resistant strains in T.asahii, which were induced by fluconazole alone,combined with methylprednisolone, and methylprednisolone alone. Methods 16clinical and environmentalstrains ofT.asahiiwere tested the susceptibility againstfluconazole in vitro, and wereinduced resistance to fluconazole by fluconazole alone,fluconazole combinated with methylprednisolone and methylprednisolone alonerespectively,comparing with the control group.The resistant strains were passed 30 times in drug-free medium to assess the stability of resistance.Results The negative control?group and?methylprednisolonealone group?had no obvious?changes in? MIC during the induction.12 resistant strains(75%) were obtained by?fluconazole?alone induction group, and 16 strains(100%)?were obtainedbyfluconazolecombinedmethylprednisolone?group.After 30?passages,?flucona