大孔吸附树脂对草鱼蛋白水解液脱盐的作用-食品科学.PDF

84 2016, Vol.37, No.05 食品科学 ※基础研究 大孔吸附树脂对草鱼蛋白水解液脱盐的作用 * 陈丽丽，赵 利 ，袁美兰，白春清，冯 飞，王 璐 （江西科技师范大学生命科学学院，国家大宗淡水鱼加工技术研发分中心，江西省生物加工过程重点实验室，江西南昌 330013 ） 摘  要：为了脱除草鱼蛋白水解液中的盐，本研究筛选了4 种型号大孔吸附树脂DA201-C 、DA201-M 、SQT-67 和D002 ，对水解液中草鱼多肽的吸附能力进行测定，并进行静态吸附和解吸实验。结果表明：大孔吸附树脂 DA201-C对草鱼多肽的吸附效果较好，其吸附量随草鱼多肽质量浓度的增加而增加；体积分数75%的乙醇对大孔吸 附树脂DA201-C 的洗脱效果最佳；DA201-C大孔吸附树脂对水解液脱盐的最佳条件为上样流速0.5 BV/h ，上样质量 浓度为30 mg/mL，水清洗速率为2 BV/h，洗脱采用体积分数75%的乙醇以2 BV/h 的流速洗脱，在此条件下大孔吸附 树脂DA201-C对水解液的脱盐率达97.17%。 关键词：草鱼；蛋白水解液；大孔吸附树脂；吸附；解吸 Desalination of Grass Carp Protein Hydrolysate Using Macroporous Adsorption Resin * CHEN Lili, ZHAO Li , YUAN Meilan, BAI Chunqing, FENG Fei, WANG Lu (Jiangxi Province Key Laboratory for Bioprocess Processing, National RD Branch Center for Freshwater Fish Processing, College of Life Science, Jiangxi Science and Technology Normal University, Nanchang 330013, China) Abstract: In order to remove salt from grass carp protein hydrolysate, four macroporous adsorption resins including DA201-C, DA201-M, SQT-67 and D002 were selected for the highest adsorption capacity for grass carp peptides through static adsorption and desorption experiments. The results showed that DA201-C had the best adsorption efficiency for grass carp peptides. Its adsorption quantity increased with increasing concentration of grass carp peptides. Seventy-five percent ethanol revealed the best elution efficiency for DA201-C. The optimal conditions for the desalination of grass carp peptides with DA201-C were determined as follows: the sample at 30 mg/mL was passed through the column at a flow rate 0.5 BV/h, and then the column was washed with at a flow rate of 2 BV/h and eluted with 75% ethan