荧光分析描述方法好文章Hydrogenperoxideproductionisanearlyeventduring(1).pdf

荧光分析描述方法好文章Hydrogenperoxideproductionisanearlyeventduring(1).pdf

  1. 1、本文档共9页,可阅读全部内容。
  2. 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
  3. 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载
  4. 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
查看更多
Planta (2007) 225:1421–1429 DOI 10.1007/s00425-006-0450-6 ORIGINAL ARTICLE Hydrogen peroxide production is an early event during bicarbonate induced stomatal closure in abaxial epidermis of Arabidopsis V. A. Kolla · A. Vavasseur · A. S. Raghavendra Received: 16 July 2006 / Accepted: 6 November 2006 / Published online: 8 December 2006 © Springer-Verlag 2006 Abstract The presence of 2 mM bicarbonate in the Abbreviations incubation medium induced stomatal closure in abaxial ABA Abscisic acid epidermis of Arabidopsis. Exposure to 2 mM bicarbon- DPI Diphenylene iodonium ate elevated the levels of H2O2 in guard cells within H2DCF-DA DichloroXuorescein diacetate 5 min, as indicated by the Xuorescent probe, dichloro- MJ Methyl jasmonate Xuorescein diacetate (H2DCF-DA). Bicarbonate- PI3P Phosphatidylinositol 3-phosphate induced stomatal closure as well as H2O2 production ROS Reactive oxygen species were restricted by exogenous catalase or diphenylene iodonium (DPI, an inhibitor of NAD(P)H oxidase). The reduced sensitivity of stomata to bicarbonate and Introduction H2O2 production in homozygous atrbohD/F double mutant of Arabidopsis conWrmed that NADP(H) oxi- Stomatal movements are brought out through volume dase is involved during bicarbonate induced ROS pro- changes in guard cells in response to various factors duction in guard cells. The production of H2O2 was such as light, temperature, CO2, or phytohormones quicker and greater with ABA than that with bicarbon- (Willmer and Fricker 1996). The stomatal opening is ate. Such pattern of H2O2 production may be one of stimulated by ligh

文档评论(0)

wx171113 + 关注
实名认证
内容提供者

该用户很懒,什么也没介绍

1亿VIP精品文档

相关文档