宏条形码技术在食品物种鉴定中的应用及展望.PDFVIP

280 2018, Vol.39, No.13 食品科学 ※专题论述 宏条形码技术在食品物种鉴定中的应用及展望 邢冉冉，吴亚君，陈 颖* （中国检验检疫科学研究院，北京 100176 ） 摘  要：食品物种鉴定是食品真伪鉴别中的重要研究内容之一。聚合酶链式反应、基因芯片等方法均被应用于食品 物种鉴定。近几年，基于高通量测序的宏条形码技术发展迅速，与基于传统测序方法的条形码技术相比，该技术具 有成本低、通量高、速度快等优点，且可以实现同时检测复杂样品中多个物种的目的，因而在食品物种鉴定方面显 现出很大的优势。本文介绍了宏条形码的含义和研究方法，综述了近年来宏条形码技术在食品物种鉴定领域的应 用，总结和讨论了宏条形码技术应用于食品物种鉴定领域面临的挑战，并对该技术的发展方向进行了展望。 关键词：DNA条形码；高通量测序；宏条形码；食品物种鉴定 DNA Metabarcoding in Food Species Identification: Current Applications and Future Prospects * XING Ranran, WU Yajun, CHEN Ying (Chinese Academy of Inspection and Quarantine, Beijing 100176, China) Abstract: Food species identification is one of the most important aspects in food authenticity assessment. Polymerase chain reaction and gene chip technology have been applied for food species identification. High-throughput sequencing- based metabarcoding technology has developed rapidly in recent years. Compared with the standard Sanger sequencing- based DNA barcoding approach, metabarcoding has shown great advantages in food species identification due to its low cost, high throughput and rapidity for the simultaneous detection of multiple species in complex samples. This review presents the definition of metabarcoding and the methods used in this research field. Then, we summarize the applications and challenges of metabarcoding in food species identification. Finally, we discuss the future direction for the development of metabarcoding. Keywords: DNA barcoding; high-throughput sequencing; metabarcoding; food species identification DOI:10.7506/spkx1002-6630-201813042 中图分类号：TS207.3 文献标志码：A 文章编号：1002-