SH-SY5Y细胞帕金森病的体外多巴胺能细胞模型解洪荣1胡林森1.DOCVIP

SH-SY5Y细胞：帕金森病的体外多巴胺能细胞模型 解洪荣1, 胡林森1, §和李国毅1 目的 综述SH-SY5Y细胞做为多巴胺能神经元细胞模型用于PD研究的潜力以及分化对这个细胞模型的影响。 结果 因为SH-SY5Y细胞具有许多多巴胺神经元的特征，包括表达多巴胺-β-羟化酶和酪氨酸羟化酶以及多巴胺转运体活性，已被广泛用做PD研究的多巴胺能神经元细胞模型。SH-SY5Y细胞在不同分化因子的作用下能够分化成有功能的成熟神经元表现型，同时分化使细胞停止增值并在整个实验中提供稳定的细胞数。然而不同的分化因子诱导不同的神经元表现型和生化改变。例如维甲酸诱导胆碱能神经元分化表现型并增加SH-SY5Y细胞对神经毒性和神经保护的耐受性，而维甲酸伴随佛波酯处理使SH-SY5Y细胞分化成多巴胺能神经元表现型并降低细胞的耐受性。某些分化因子还能改变SH-SY5Y细胞摄取MPP+的动力学，从而使细胞与体内和原代培养的中脑神经元具有更大的相似性。 结论 未分化和分化的SH-SY5Y细胞都被广泛的用做PD研究的多巴胺能细胞模型。某些分化因子使SH-SY5Y细胞在神经毒性、神经保护性和实验性PD研究中更具有潜力。 关键词：帕金森病；SH-SY5Y细胞；细胞模型；分化；多巴胺能神经元。 SH-SY5Y human neuroblastoma cell line: in vitro cell model of dopaminergic neurons critical in Parkinson’s disease XIE Hong-rong1, HU Lin-sen1, § and LI Guo-yi1 1Department of Neurology, the First Hospital, Jilin University, Changchun 130021, China §Correspondence to: Linsen Hu, M.D., Ph.D. Department of Neurology, the First Hospital, Jilin University, 1 Xinmin Street, Changchun 130021, P. R. China Phone: +86-431Fax: +86-431-5637090 Email: hulinsen@ Key words: Parkinson’s disease; SH-SY5Y cells; cell model; differentiation; dopaminergic neuron Objective To evaluate the human neuroblastoma SH-SY5Y cell line as an in vitro model of dopaminergic (DAergic) neurons for Parkinson’s disease (PD) research and to determine the effect of differentiation on this cell model. Results This paper will review the use of the human neuroblastoma cell line SH-SY5Y as a cell model of DAergic neurons for PD research. The SH-SY5Y cell line has become a popular cell model for PD research because this cell line posses many characteristics of DAergic neurons. For example, these cells express tyrosine hydroxylase and dopamine-β-hydroxylase, as well as the dopamine transporter. Moreover, this cell line can be differentiated into a functionally mature neuronal phenotype in the presence of various agents. Upon differentiation, SH-SY5Y cells stop proliferating and a constant cell number is subsequently maintained. However, different differentiating agents