HPLC法比较不同产地夏枯草属药材中成分组成的差异性.PDFVIP

# 1384 # 中草药 Chinese Traditional and Herbal Drugs 41 8 2010 8 HPLC 1, 2 1, 2* 1 1 1 1 1 刘光敏 , 贾晓斌 , 陈 彦 , 朱粉霞 , 封 亮 , 蒋 俊 , 施 峰 ( 11 , , 210028; 21 , 212013) : , HPLC 9 3 , P runella v ulg ar is L. , P . asiat ica Nakai, P . hisp ida Bent , ; 4 ; 4 , 11 0B 141 7 B 31 9B 11 0 11 0B 141 8B 41 0B 01 8 , ; , : ; ; ; ; ; ; ; HPLC : R2861 1 :A : 0253 2670( 2010) 08 1384 03 P rune ll a v ulg aris L. , , , P rune ll a hisp i da Bentham, , 2005 P . v ulg ar is L. , [ 1] 56 P . as iatica Nakai [ 2] , 4 3 , 2 , 21 1 : A lltima C18 ( 250 mm @ 4. 6 mm, 5 Lm) , 350 nm, 30 e , 20 LL (A) 01 1% [ 3 4] [ 5] , , ( 0 ~ 10 min, 25% ~ 40% A; 10 ~ 60 min, HPLC 9 3 40%~ 60% A; 60~ 70 min, 60% ~ 60% A) Pr unel l a v ulg ar is L. , P . as i- 70 min 1 mL/ min atica Nakai, P . hisp i da Bentham 21 2 , 21 21 1 : , , 60% [ 6] 771 28 Lg/ mL 1 84196 Lg/ mL 2381 00 Lg/ mL Agilent 1200 :DAD 301 60 Lg/ mL 1 mL , ; BP ) 211D ( Sar torius , ) (T EDIA, 21 212 : 015 g, ) , , 50 mL 60% ( 2 , 2 h/ ) , , , , 60% ( 10 mL, ( 14 000 r/ 080420, \98%) min, 15 min) , , 21 3 : * : 20 09 10 3 0 : ( BK2006155) ( 2006, 2009) : ( 1985)