COX-2抑制剂联合放疗对人乳腺癌裸鼠移植瘤作用的实验分析.pdfVIP

合组 0.833±0.236g，差异有显著意义（P0.01 ）。 3 3. 治疗结束后，各组种植瘤体积分别为：对照组 1124.333±41.362 mm ；塞 3 3 来昔布组 996.500±33.066 mm ；放疗组977.750±30.896 mm ；塞来昔布联合放疗 3 组 932.416±63.922 mm ，差异有显著意义（P0.01 ）。 4. 各组裸鼠移植瘤的免疫组化结果显示：COX-2 、VEGF 及 MMP-9 在对照 组中大多为阳性表达，在塞来昔布组和放疗组中表达稍有下降，而塞来昔布联合 放疗组中表达下降更为显著(P0.05) 。 结论 1. 塞来昔布联合放疗抑制人乳腺癌 MDA-MB-231 裸鼠移植瘤明显优于单 纯药物组及单纯放疗组。 2. 塞来昔布增强人乳腺癌裸鼠移植瘤放疗敏感性的机制可能为：1）降低了 VEGF 的表达。2 ）塞来昔布通过阻断MMP-9 的表达。 关键词 乳腺癌；放疗； COX-2 ；VEGF ；MMP-9 3 Experimental study of effects of COX-2 inhibitor combined with radiotherapy on xenograft tuomor of breast cancer in nude mice ABSTRACT Objectives To establish the breast cancer xenograft tuomor mode on nude mice, detect the expression of COX-2, VEGF and MMP-9 after given celecoxib.To study whether celecoxib can enhance their radiation sensitivity,and the mechanism of the anti-tumor effect, to find a promising radiosensitization drug,and clarify the theoretical basis. Method Select 4 to 5 weeks old female nude mice 48 , Select the human breast cancer MDA-MB-231 cells in logarithmic phase , configured out a 7 density of 1×10 /ml cells .After skin degerming,inject 0.2ml the solution by 1ml syringe on back of the nude mice to establish a xenograft animal model. Measure the tumor by sliding caliper regularly, When tumor diameter of 5mm or so long to carry out experiment. Two weeks later 48 mice were randomly divided into four groups : control group (A group), celecoxib group (B group), radiotherapy group(C group), radiotherapy combined celecoxib group(D group). In group A, each nude mice were feed with NS(normal sa