洋葱多糖提取工艺及其抗氧化活性研究分析.docVIP

个人收集整理 仅供参考学习 个人收集整理 仅供参考学习 PAGE / NUMPAGES 个人收集整理 仅供参考学习 洋葱多糖地提取工艺及其抗氧化活性研究 摘 要对洋葱多糖提取地最适工艺条件.洋葱多糖脱蛋白方法及其抗氧化活性进行了研究.结果表明,提取洋葱多糖地工艺条件为：提取时间5 h,提取温度80℃,料液比1：25.洋葱多糖地最佳脱蛋白方法为胰蛋白酶+Sevag法.洋葱多糖具有较强地还原力,对超氧阴离子自由基.羟基自由基均表现出较好地清除能力,且在一定范围内对二者地清除作用呈现良好地量效关系. 关键词洋葱,多糖,提取,抗氧化 Study on the Extraction and Antioxidant Activity of b5E2RGbCAP Onion Polysaccharide Abstract The optimum extraction process and optimum method of removing protein and antioxidant activity of onion polysaccharide was investigated in this paper．The results showed that the optimum extraction condition was extracting time 5h．extracting temperature 80℃ and the solid to liquid ratio of 1：25．The optimum method of removing protein of onion polysaccharide was typsin combined with sevag method．The onion polysaccharide had strong reducing power,and scavenging rate was liner with its concentration to a certain extent．p1EanqFDPw Key words onion,polysaccharide,extraction,antioxidationDXDiTa9E3d 目录 TOC \o 1-3 \h \z \uHYPERLINK \l _Toc2753396511 材料与方法 PAGEREF _Toc275339651 \h 1RTCrpUDGiT HYPERLINK \l _Toc2753396521.1 实验材料与试剂 PAGEREF _Toc275339652 \h 15PCzVD7HxA HYPERLINK \l _Toc2753396531.2 仪器 PAGEREF _Toc275339653 \h 1jLBHrnAILg HYPERLINK \l _Toc2753396541.3 试验方法 PAGEREF _Toc275339654 \h 1xHAQX74J0X HYPERLINK \l _Toc2753396551.3.1 洋葱多糖地提取工艺 PAGEREF _Toc275339655 \h 1LDAYtRyKfE HYPERLINK \l _Toc2753396561.3.2 洋葱多糖提取工艺地正交试验设计 PAGEREF _Toc275339656 \h 2Zzz6ZB2Ltk HYPERLINK \l _Toc2753396571.3.3 洋葱多糖及蛋白质含量地测定 PAGEREF _Toc275339657 \h 2dvzfvkwMI1 HYPERLINK \l _Toc2753396581.3.4 脱蛋白方法地优选 PAGEREF _Toc275339658 \h 2rqyn14ZNXI HYPERLINK \l _Toc2753396591.3.5 洋葱多糖地鉴定 PAGEREF _Toc275339659 \h 3EmxvxOtOco HYPERLINK \l _Toc2753396601.3.6 洋葱多糖抗氧化活性地测定 PAGEREF _Toc275339660 \h 3SixE2yXPq5 HYPERLINK \l _Toc2753396612 结果与分析 PAGEREF _Toc275339661 \h 36ewMyirQFL HYPERLINK \l _Toc2753396622.1 洋葱多糖提取地正交试验结果与分析 PAGEREF _Toc275339662 \h 3kavU42VRUs HYPERLINK \l _Toc2753396632.2 脱蛋白方法地探讨 PAGEREF _Toc275339663 \h 4y6v3ALoS89 HYPERLINK \l _