质谱讲义4-4(解析-串联)..ppt

第四节、 串联质谱应用 一、碰撞诱导裂解（CAD） 二、源内CAD 三、IRMPD和ECD 一、碰撞诱导裂解（CAD） 二、源内CAD 三、IRMPD和ECD Infrared Multiphoton Dissociation: IRMPD Electron Capture Dissociation: ECD ECD 9 eV [M+nH]n+ + e- [M+nH](n+1)+· + 2e- [M-nH]n- + e- [M-nH](n-1)-· + 2e- [M-nH]n- + e- ([M-nH](n-1)-·)*transient + 2e- fragments + e- More energetic electrons may cause double ionization and Coulombic explosion. * API 4000 LC/MS/MS APCI ESI Linear Varian 1200 GC/MS/MS 180o Magnet ESI source capillary ion guide cell Differential pumping stages CO2 - Laser HP