利用原位杂交及CAPS标记分析芸薹属AB和C基因组间-CiteSeerX.PDF

作物学报 ACTA AGRONOMICA SINICA 2008, 34(7): 1188−1192 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2008.01188 利用原位杂交及CAPS 标记分析芸薹属A 、B 和C 基因组间的关系 孔 芳 蒋金金 吴 磊 王幼平* (, 225009) : Brassica rapa (AA)(151 bp), (AA, 2n=20) (CC, 2n=18)(AABB, 2n=36), , 20 , , A B 3 3 FAE1 CAPS , 3 , Mbo I Msp I , A C , B AC , 3 2 , : ; ; ; CAPS ; Relationships among Genome A, B, and C Revealed by FISH and CAPS * KONG Fang, JIANG Jin-Jin, WU Lei, and WANG You-Ping (College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, Jiangsu, China) Abstract: Physical localization of repetitive DNA sequence from genome A (151 bp) was carried out on the chromosomes of the selected Brassica species by FISH (fluorescence in situ hybridization). The signals distributed on all the chromosomes of A (Brassica rapa, 2n=20) or C genome (B. oleracea, 2n=18). For B. juncea (AABB, 2n=36), the signals were found on all the chromosomes of genome A and the strength of signal varied among different chromosomes, while the chromosomes of genome B showed weak or no signals. FAE1 gene is a rate-limiting gene for erucic acid synthesis in Brassica . The genes from six Brassica species of U-triangule were cloned by PCR. These PCR products were digested with different restriction endonucleases. Mbo I and Msp I were found to produce informative CAPS patterns of FAE1 gene. Three diploids displaye