半边旗抗肿瘤有效成分对HL-60细胞DNA拓扑异构酶活性及其细胞.PDF

ISSN㈣ 昕56 ActaPhannacolSin 中国药理学报 l邺 Jun；20(6)：541—546 ：／， slmm 脚 ； www Clli~infog 口¨∞口。 Effectsofantitumorcompoundsisolatedfrom Pterissemipinnata L onDNA topoisomerasesandcellcycleofHL·60cells1 LIJin-Hua2，HECheng-Wei，LIANGNian-Ci，MOLi一 ，ZHANGXiao (InstituteofMedicalBiochemistry DepartmentofChemistry，GuangdongMedicalCollege，Z~mjiang524023，China) KEY WORDS Pterfssemipbmata L：diterpenez； enhna ced the cytotoxicity ofgenisotin， which had DNA otpoisornemse； cellcycle； genistein； HL-60 synergism wiht 6Fni natiamaoraction． cells；drugsynergism ；antineoplasticagents Pterissemipinnata L (PsL)，aChniesetradi- AⅡⅡ：T0study 山eeffectof山eantitumorcompounds tionalherbwildlydistributedinSouhtern China．isused 5F．6F．and A from Pt is s州 iF’0／ru2ta L oN me to treathepatitis．enteritis．and snak~biteL n e activities ofDNA topo isornerasesand celleyele of ethano ficextractof山e whole plnatof L had aI1 HI_60 cells．nadthe synergism ofcompound6F iII natitumoractivity ． Oursutdieshave shown that combination wi山 genistein in vitro． TⅡETHODS． constiutents isohtedfrom PsLhadastrongcytotoxicity DNA topoisomeraseswere isolated from HL-60 cell againsthumanpmmyelocyti~leukemiacells(r~-60)， lines． and superociled pBR322 DNA was used as gastric adenocarcnioma cellMGC-803， lung adeno- substra~ to deterro．m~ the activities of DNA carcinoma cell line SPC-A-1， nasopharyngeal otpoisomeraseInad11．Cel1cyclewasnaalyzdeby carcinomacelllineni low differentiation (CNE-2Z)， flowcy~metry(F(：M)．Cytotoxicityassaywastested liveradennoarcinoma eellline BEL7402．and }IePG byMTrmethod． REslI1 ：Compoun ds 5F，6F， 11【． andA inhibited山eacfivltiesofDNAtopoisomeraseI DNA topo isomemses ILre enzyme~ thatalterhte nadⅡ Afterexposureofhtecellstocompound6F， topo logicalstructureofDNA by sWand breakageand aI1incre,a,se in cellsin山eSan