若尔盖高原产甲烷菌数量的时空差异性-微生物学报.PDF

微生物学通报 NOV 20, 2010, 37(11): 1706−1711 Microbiology China © 2010 by Institute of Microbiology, CAS tongbao@ 主编点评文章 若尔盖高原产甲烷菌数量的时空差异性 汤博 唐杰 吴俐莎 杨志荣 张杰* ( 四川大学生命科学学院 生物资源与生态环境教育部重点实验室 四川 成都 610064) 摘 要: 应用实时荧光定量PCR 法, 构建了总甲烷菌实时荧光绝对定量PCR 的标准品以及标准曲 线, 用于总甲烷菌的定量测定, 所构建的质粒DNA 浓度为160 mg/L, 标准曲线相关性为0.992, 扩 增效率为 98.6% 。研究表明, 若尔盖花湖地区4 月草地生态系统和湿地生态系统甲烷菌含量相当, 湿地生态系统9 月甲烷菌含量高于7 月, 7 月甲烷菌含量高于4 月, 在7 月和9 月随着深度的增加 甲烷菌含量也增加, 而草地生态系统的甲烷菌含量并未表现出明显的季节和深度上的规律性。 关键词: 若尔盖高原, 实时荧光定量PCR, 甲烷菌 Time-space Change of Methanogen Amount from Zoige Plateau * TANG Bo TANG Jie WU Li-Sha YANG Zhi-Rong ZHANG Jie (College of Life Sciences, Key Laboratory of Bio-resource and Bio-environment, Sichuan University, Chengdu, Sichuan 610064, China) Abstract: To quantify the population size of total methanogens changing with environments, season and depths of Zoige plateau, the plasmids and standard curves of species were constructed by using real - time absolute quantitative PCR. The concentration of the standard plasmids was 160 mg/L, with the coef- ficient being 0.992, and the amplification efficiency was 98.6%. The results showed that in April the population size of total methanogens in wetland ecosystem and grassland ecosystem were nearly the same. In wetland ecosystem, the population size of total methanogens decreased in the order of September, July, and April,