苦参碱对表皮葡萄球菌生物被膜作用初探-微生物学报.PDF

微生物学通报 JAN 20, 2011, 38(1): 91−96 Microbiology China © 2011 by Institute of Microbiology, CAS tongbao@ 简 报 苦参碱对表皮葡萄球菌生物被膜作用初探 * 官妍 马越 程惠娟 汪长中 谢萌 张爽 王艳 ( 230038) 摘 要: 通过中药有效成分苦参碱对表皮葡萄球菌生物被膜抑制作用的研究, 为表皮葡萄球菌生 物被膜引起的相关感染提供新的治疗途径。利用XTT 减低法评价苦参碱对表皮葡萄球菌初始粘附 及生物被膜内细菌代谢的影响, 镜下观察该药对表皮葡萄球菌生物被膜的形态学影响。结果表明: 苦参碱对表皮葡萄球菌生物被膜菌的SMIC50 和SMIC80 分别为62.5 mg/L 和500 mg/L; 1 000 mg/L 浓度的苦参碱对表皮葡萄球菌早期粘附有抑制作用; 250 mg/L 浓度的苦参碱对表皮葡萄球菌生 物被膜的形态有显著影响。因此可见, 苦参碱对表皮葡萄球菌生物被膜的形成与粘附均有抑制 作用。 关键词: 生物被膜, 表皮葡萄球菌, 苦参碱 Preliminary study of effects of matrine on Staphylococcus epidermidis biofilms * GUAN Yan MA Yue CHENG Hui-Juan WANG Chang-Zhong XIE Meng ZHANG Shuang WANG Yan (Anhui University of Traditional Chinese Medicine , Hefei, Anhui 230038, China) Abstract: A new therapeutic approach for Staphylococcus epidermidis biofilm-associated infections was provided by the study of active ingredient of Chinese medicine matrine on S. epidermidis biofilm inhibition. XTT reduction assay was used to evaluate matrine on the initial adhesion of S. epidermidis and biofilm metabolism, and microscope was applied to observe biofilm morphology. The results showed that SMIC50 and SMIC80 of matrine against S. epidermidis biofilms were 62.5 mg/L and 500 mg/L respec- tively, 1 000 mg/L of matrine inhibited the early adherence of S. epidermidis, and 250 m