免疫共沉淀技术验证蛋白质相互作用的研究翟华立.ppt

免疫共沉淀技术原理 Co-Immunoprecipitation(CO-IP) a classical method used for research protein-protein interaction on the basis of specificity of antibody to antigen. an effective method to confirm two kinds of proteins endogenous interaction in cell. It utilizes antibody to precipitate the corresponding specific molecule meanwhile the other molecules with specific binding to the molecule will be precipitated. be used to validate proteins specific binding. 免疫共沉淀技术原理 基本原理是：在非变性条件下，将细胞裂解，细胞裂解液中加入抗目的蛋白的抗体，与抗原形成特异免疫复合物，经过洗脱，收集免疫复合物，然后进行SDS及Western blotting分析. 免疫共沉淀技术原理图解 hCLP46与caln