新颖肿瘤标志物GW112在胃癌及结直肠癌等消化道.doc

GW112在食管鳞癌中的表达研究 Expression of GW112 in esophageal squamous cell carcinoma 汪令伟 王林 作者单位：116021 辽宁省大连市沙河口区万岁街156号 大连大学附属新华医院 通信作者：王林，Email：HYPERLINK mailto:wanglinbox@wanglinbox@ 简介：王林，男，主任医师，医学博士，教授，硕士生导师. 汪令伟，男，硕士研究生. 汪令伟：电话 Email： HYPERLINK mailto:416758600@ 416758600@ 基金项目：辽宁省大连市科技计划项目(2012E15SF147) 【摘要】 目的：检测新颖肿瘤标志物GW112基因在食管鳞癌中的表达情况，以期促进对食管鳞癌的肿瘤标志物的研究。方法：用免疫组织化学法检测对70例食管癌组织和正常食管组织中的GW112蛋白表达；TaqMan探针荧光定量逆转录聚合酶链反应（QRT-PCR)方法检测26例食管鳞癌及正常食管组织中GW112基因的mRNA的表达水平；对获得的实验数据结合标本的临床资料进行统计学分析。结果：TaqMan探针荧光定量PCR和免疫组化结果表明，在正常食管组织中无GW112基因的表达；GW112基因在食管鳞癌组织中表达水平上调；GW112基因表达与年龄、临床TNM分期、肿瘤分化程度以及淋巴结转移情况相关，并且在高龄、临床TNM中晚期、中低分化以及存在淋巴结转移的食管鳞癌组织中GW112基因的表达上调更为明显。结论：抗凋亡基因GW112在食管鳞癌中表达上调，食管鳞状细胞癌的发生发展中显然GW112基因表达水平的上调起着重要的作用。 【Abstract】 Objective:GW112 (also called olfm4 and hGC-1) is a gastrointestinal protein that is a member of the olfactomedin glycoprotein family. Aberrant expression of GW112 in some human carcinomas has been recently reported, especially in gastric carcinoma and Colon Carcinoma. The purpose of this study was to examine GW112 expression in esophageal squamous cell carcinoma and explore the relationship between GW112 expression and the clinicopathologic features of patients with esophageal squamous cell carcinoma (ESCC). Methods:The expression of GW112 in esophageal squamous cell carcinoma tissues was examined by real-time reversetranscription polymerase chain reaction (Real-time PCR) and immunohistochemistry. The association of GW112 expression pattern with patient differentiation grade, tumor stage, age and Lymph node metastasis were examined. Results: Compared with normal Esophageal tissue, the up-regulation of GW112 was more frequently detected in esophageal squamous cell carcinoma. Interestingly, GW112 up-regulation was also more frequently found in old, poorly differentiation, advanced and lymph node-metastasis stage. Conclution: Our study indicate that the expression GW112 is associated with old peo