信息检索第五次作业、.docVIP

《文献信息检索》综合作业3 要求： 1．通过实习，巩固所学的检索策略及资源类型。 2．写出所用检索词、检索表达式及命中篇数并复制粘贴2篇在文挡中。 3．课堂作业评点和分析时，希望积极参与教学互动。 一、医学数据库检索 1、Pubmed库 （1）检索福建中医药大学吴锦忠教授发文情况，分别以题录（Summary）和文摘（Abstrats）格式下载保存。 答案：检索式：Wu, Jinzhong[Full Author Name] 检索结果：3 文摘方式： 1. J Nat Med. 2013 Jan;67(1):17-26. doi: 10.1007/s11418-012-0639-x. Epub 2012 Mar 1. Investigation of the extracts from Bidens pilosa Linn. var. radiata Sch. Bip. for antioxidant activities and cytotoxicity against human tumor cells. Wu J, Wan Z, Yi J, Wu Y, Peng W, Wu J. Fujian University of Traditional Chinese Medicine, Fuzhou, Peoples Republic of China. The aim of this study was to evaluate antioxidant activities and cytotoxicity against human tumor cell lines of extracts from Bidens pilosa Linn. var. radiata Sch. Bip. (BP). Total phenolic and flavonoid contents of the extracts were determined by ultraviolet spectrophotometry. Furthermore, the antioxidant properties of different polarity fractions extracted from BP were evaluated using DPPH and ABTS radical scavenging test and FRAP assay. The ethyl acetate fraction (EE-BP) showed the highest antioxidant activity compared to other fractions. In addition, the anti-proliferative activities of the extracts on four human tumor cells, namely MCF-7, HepG2, MGC 803 and RKO, were investigated by MTT method. The EE-BP displayed the most remarkable anti-proliferative effect against the tumor cells, particularly RKO cell in dose- and time-dependent manner. The antioxidant activities and cytotoxicity correlated highly with the total phenolic and flavonoid contents, respectively. Furthermore, The active ingredient BP-6, namely 5,7,4-trihydroxy-3,3-dimethyl-flavonol, was isolated and purified with the purity above 99.00% and content of 0.15% in EE-BP detected by HPLC, which could significantly inhibit the proliferation of RKO cells with the IC(50) value of 6.66?μmol/l. In order to characterize the apoptotic RKO cells, flow cytometry and DNA fragmentation assay were performed. Apoptotic cell numbers inc