芍药苷对大鼠局灶性脑缺血再灌注脑血流量及.doc

PAGE \* MERGEFORMAT PAGE \* MERGEFORMAT 11 芍药苷激活CB2受体对脑缺血神经的保护作用 蔡江晖，饶梦琳，唐蜜，向丽，封莎，肖成燕，刘颖菊* （重庆医科大学药学院生化与分子药理研究室，重庆 400016） 摘要：目的 探究芍药苷通过调节二型大麻受体（CB2）对脑缺血再灌注大鼠神经的保护作用。方法 120只雄性SD大鼠随机分为假手术组、模型组、溶媒组、芍药苷低、高（ 10mg kg-1、 40mg kg-1）剂量组、CB2选择性拮抗剂AM630组、芍药苷高剂量组+AM630组、CB2选择性激动剂HU308组。线栓法制作大鼠局灶性脑缺血(90min)/再灌注(24h)模型。观察芍药苷对神经功能缺损评分、梗塞体积、脑水肿及脑组织病理形态学的影响；采用免疫组化法研究芍药苷对Casepase-3和COX-2表达的影响。结果 芍药苷能显著改善脑缺血大鼠神经缺损症状，降低梗塞体积及缺血侧脑含水量，改善脑组织病理形态学改变，抑制Casepase-3和COX-2表达；但预先注射AM630可显著拮抗芍药苷的脑神经保护作用。结论 二型大麻受体可能参与芍药苷的抗脑缺血神经保护作用。 关键词:脑缺血再灌注；芍药苷；二型大麻受体；神经保护作用 [中图分类号] R965 [文献标识码] A 作者简介：蔡江晖，男，硕士研究生，主要从事免疫药理方面的研究，Phn: 86E-mail:776773221@； 通讯作者：刘颖菊，女，教授，硕士生导师，主要从事神经药理与免疫药理方面的研究，E-mail：2553407160@ Neuroprotective effects of paeoniflorin on cerebral ischemic rats by activating cannabinoid 2 receptors CAI Jiang-hui, RAO Meng-lin, TANG Mi, XIANG Li, FENG Sha, XIAO Cheng-yan, LIU Ying-ju* (Teaching and Research Section of Biochemical and Molecular Pharmacology, College of Pharmacy of Chongqing Medical University, Chongqing 400016, China) ABSTRACT: AIM To investigate neuroprotective effects of paeoniflorin on cerebral ischemic rats by regulating cannabinoid 2 receptors. METHODS 120 maleSD rats were randomly divided into 8 groups:Sham operation group, cerebral ischemia-reperfusion model group, vehicle control group, low（10mg kg-1）and high（40mg kg-1）doses of paeoniflorin groups, selective CB2 receptor antagonist AM630 group , paeoniflorin (40mg kg-1) with AM630 group, and selective CB2 receptor agonist HU308 group. Focal cerebral ischemia in rats were made by inserting a monofilament suture into internal carotid artery for 90min and then reperfused for 24 h. The effects of paeoniflorin on neurological neurological deficit scores, the infarction volume and cerebral edema were detected. Pathological changes were observed by HE stain and the expression of Casepase-3 and COX-2 in hippocampal CA1 region were determined by immunohistochemistry . RESULTS Paeoniflorin co