柴胡加龙骨牡蛎汤对SPS建立的PTSD样大鼠行为-中国动物学报.DOCVIP

孤养对SPS单次延长应激建立的PTSD创伤后应激障碍模型大鼠行为学的影响 基金项目：国家自然科学基金青年科学基金项目；国家级大学生创新创业训练项目（2017100126030） 作者简介：#为共同第一作者 方锦颖，（1995-）女，八年制本科阶段，专业：中医学，E-mail：fangjinying1@163.com 陈光耀（1994-），男，研究生在读，专业：中医内科学，E-mail：chenguangyao1994@163.com。 通讯作者简介：毛萌（1985-），女，助理研究员，医学博士，研究方向：神经精神疾病病证结合研究E-mail：mmcior@163.com。 方锦颖# ，陈光耀# ，李龙梅 ，郑思思 ，毛萌* （北京中医药大学，北京 100029） 【摘要】 目的 通过SPS建立PTSD模型，观察孤养对SPS模型大鼠的影响。 方法 将36只大鼠随机分为正常组、SPS模型组及SPS联合孤养组，SPS模型组、SPS联合孤养组给予SPS造模，SPS联合孤养组造模后进行单独饲养。造模7天d后检测大鼠体重增长数，旷场实验中运动总距离、穿格次数以及单次最大运动距离，僵立行为测试中僵立行为次数及时间。结果 （1）与正常组大鼠进行比较，SPS模型组大鼠体重增长数、单次最大运动距离明显减少（P0.01），运动总距离、穿格次数减少（P0.05），僵立行为测试僵立行为次数及时间明显增加（P0.01）；SPS联合孤养组大鼠体重增长数减少（P0.05），僵立行为测试僵立行为次数及时间增加（P0.05）。（2）与SPS模型组比较，SPS联合孤养组旷场实验中运动总距离、穿格次数以及单次最大运动距离增加（P0.05）。结论 本实验成功通过SPS复制了PTSD大鼠模型，7天d孤养使得SPS模型焦虑状态得到改善。 【关键词】 创伤后应激障碍；孤养；单次延长应激；行为学； Behavioral changes of post-traumatic Stress Disorder model rats established by single-prolonged stress through social isolate FangFANG Jin-ying ,ChenCHEN Guang-yao ,LiLI Long-mei ,ZhengZHENG Si-si ,MaoMAO Meng* （Beijing University of Chinese Medicine，Beijing 100029，China） 【Abstract】 Objective Establishing a PTSD model to observe the effect of social isolate on SPS model rats. Methods Thirty - six rats were randomly divided into normal group, SPS model group and SPS combined social isolate group. In addition, both the rats in SPS model group and SPS combined group were given SPS model, while the rats in SPS combined with social isolate group were fed separately. The number of weight gain, the distance movement total in open field test, the crossing frequency, the max continuous distance , the freezing time test and freezing time were measured after 7 days of modeling. Results (1) Compared with the normal group, the number of weight gain and the max continuous distance of the rats in the SPS model group were significantly decreased (P 0.01), meanwhile the distance moved total and crossing frequency were decreased (P 0.05), in the freezing time test freezing frequen