坛紫菜自由丝状体对无机碳的利用-水产学报.PDF

26 5 Vol. 26, No. 5 2002 10 JOURNAL OF FISHERIES OF CHINA Oct. , 2002 : 1000- 0615( 2002) 05- 0477- 04 # # 骆其君, 裴鲁青, 潘双叶, 王 勇, 费志清 ( , 315211) : ; ; ; : Q946. 2 : A Utilization of inorganic carbon in free-living conchocelis of Porp hyra haitanensis LUO Qi-jun, PEI Lu-qing , PAN Shuang- e, WANG Yong , FEI Zhi-qing ( K ey L aborarory of Marine B iotechnology , Ning bo University , Ningbo 315211, China) Abstract: Free-living Porp hyra haitanensis conchocelis was cultured under continual light, then it was treated w ith Van, DIDS and SITS, the inhibitors of inorganic carbon utilization, to stud the mechanism of inorganic carbon utilization in the conchocelis. The results indicate that Van inhibits the utilization of inorganic carbon most, w ith a rate of 71. 3% , and the rates of DIDS and SITS are relativel lower, 42% and 35. 5% respectivel . The inhibition rate of Az is 25. 3%, which indicates the external CA is not an important part of its inorganic carbon uptake. A complementar conclusion is that the main part of inorganic carbon absorption in P. haitanensis conchocelis is through active transport of HCO - and CO . 3 2 Key words: Porp hyra haitanensis ; free-living conchocelis; inorganic carbon( Ci) ; active transport ( HCO3 - CO32- ) ( 99% ) , pH 8. 020e , HCO3 - 1. 8mmo1# L- 1 HCO - CO , CO , 10~ 12Lmo1# 3 2 2 L- 1[ 1] CO , CO , 2