激活大鼠前额叶下边缘区GABAA受体对被动回避记忆巩固-TSpace.PDFVIP

动 物 学 研 究 2013，Dec. 34(6): 589−595 CN 53-1040/Q ISSN 0254-5853 Zoological Research DOI:10.11813/j.issn.0254-5853.2013.6.0589 激活大鼠前额叶下边缘区 GABAA 受体对被动回避记忆巩固的 影响 王晓琴 1,2 ，李仙丽2,3 ，王功伍2,* 1. 云南师范大学 体育学院，云南 昆明 650500 2. 云南师范大学 生命科学学院行为与认知神经生物学实验室，云南 昆明 650500 3. 郑州中学，河南 郑州 450001 摘要：该研究采用一次性步入法测试大鼠被动回避行为，以局部EEG 功率变化作为蝇蕈醇失活前额叶下边缘区的效果指标， 观察训练期和24 h 后重测试时大鼠进入暗箱的潜伏期，旨在探讨激活前额叶下边缘区GABAA 受体，可逆性失活该区神经元 对大鼠被动回避记忆巩固过程的影响。结果发现，蝇蕈醇可显著降低大鼠前额叶下边缘区EEG 总功率，但其被动回避作业 重测试潜伏期与对照组无明显差异；即大鼠前额叶下边缘区GABAA 受体被激活，导致该区神经兴奋性可逆性降低，但不影 响被动回避记忆的巩固过程。 关键词：前额叶下边缘区；GABAA 受体；被动回避记忆巩固；EEG 功率；Sprague-Dawley 大鼠；蝇蕈醇 中图分类号：Q189 ；Q427 文献标志码：A 文章编号：0254-5853-(2013)06-0589-07 Effect of prefrontal infralimbic cortex GABAA receptor agitating on passive avoidance memory consolidation of rats Xiao-Qin WANG1,2, Xian-Li LI2,3, Gong-Wu WANG2,* 1. School of Physical Education, Yunnan Normal University, Kunming 650500, China 2. Laboratory of Behavioural and Cognitive Neurobiology, School of Life Sciences, Yunnan Normal University, Kunming 650500, China 3. Zhengzhou Middle School, Zhengzhou 450001, China Abstract: In this study, one-trial step-through task was applied to reveal the effect of prefrontal infralimbic cortex GABAA receptor agitating on passive avoidance memory consolidation in rats. Immediately after training (with shock), GABAA receptor agonist, muscimol (0.5 μg/0.25 μL) or saline (0.25 μL) was infused into the test animal’s infralimbic cortex, then, the latencies of rats stepping through the dark room were recorded. Meanwhile, local EEG power was adopted as index of the inactivation effect of muscimol. The step-through latencies of muscimol group were comparable （P0.05 ）with saline group, but their local EEG power h