木贼麻黄多糖的提取及体外抗氧化活性研究-中国现代应用药学.PDF

木贼麻黄多糖的提取及体外抗氧化活性研究  齐佳慧，韩秋菊* ，王菲，李薇，王战勇(辽宁石油化工大学，辽宁 抚顺 113001) 摘要：目的 优化木贼麻黄多糖的提取工艺，初步研究木贼麻黄多糖的体外抗氧化活性。方法 采用热水浸提法提取木 贼麻黄多糖，利用单因素试验结合响应面法对提取条件进行优化，并研究木贼麻黄多糖的体外抗氧化活性。结果 木贼 麻黄多糖的最佳提取条件为：提取时间 2.5 h ，提取温度 73 ℃，液料比25 ∶1，在此条件下多糖提取率为(5.57±0.072)%。 木贼麻黄多糖对 DPPH 自由基和羟基自由基具有较强的清除能力，IC50 分别为 703.9 μg·mL和 2 135.1 μg·mL；对超氧 阴离子自由基具有一定的清除能力，对 Fe3+有一定的还原能力。结论 该提取工艺能较好地提取木贼麻黄多糖，所得木 贼麻黄多糖具有一定的体外抗氧化能力，但活性低于同浓度的维生素 C 。 关键词：木贼麻黄；多糖；提取；抗氧化活性 中图分类号：R284.1 文献标志码：A 文章编号：1007-7693(2018)09-1342-05 DOI: 10.13748/ki.issn1007-7693.2018.09.016 引用本文：齐佳慧, 韩秋菊, 王菲, 等. 木贼麻黄多糖的提取及体外抗氧化活性研究[J]. 中国现代应用药学, 2018, 35(9): 1342-1346. Research of Polysaccharides Extraction from Ephedra Equisetina Bunge. and Its Antioxidant Activity in Vitro * QI Jiahui, HAN Qiuju , WANG Fei, LI Wei, WANG Zhanyong(Liaoning Shihua University, Fushun 113001, China) ABSTRACT: OBJECTIVE To optimize the extraction condition of the polysaccharides from Ephedra equisetina Bunge. and study its antioxidant activity in vitro. METHODS The polysaccharides were extracted from Ephedra equisetina Bunge. by hot water extraction. The single factor tests and response surface methodology were used to optimize the extraction condition. The antioxidant activity in vitro was evaluated by artificial free radicals in detail. RESULTS The optimum extraction condition was: extraction time 2.5 h, extraction temperature 73 ℃ , ratio of liquid to material 25 ∶1. Under the optimum condition, the yield was (5.57±0.072)%. The antioxidant activity in vitro showed that the polysaccharide from Ephedra equisetina Bunge. had a better scavenging effect on DPPH radical and hydroxyl radical with IC50 of 703.9 μg·mL and 2 135.1 μg·mL respectively, and had a lower scavenging effect on s