壮药毛冬青配方颗粒一测多评的定量研究-中国现代应用药学.pdfVIP

壮药毛冬青配方颗粒一测多评的定量研究  * 农新维，梁冰丽，李颖，黄雄梅，韦红言 (培力(南宁)药业有限公司，南宁 530007) 摘要：目的 建立一测多评法同时测定毛冬青配方颗粒中 6 种酚酸类成分的含量。方法 以绿原酸为内参物，分别建立 其与隐绿原酸、咖啡酸、异绿原酸 B 、异绿原酸 A 、异绿原酸 C 的相对校正因子，并利用该相对校正因子计算含量；同 时利用外标法测定该 6 种酚酸的含量，比较 2 种测定方法的差异，验证一测多评法的准确性和可行性。结果 相对校正 因子的重现性良好，8 批毛冬青配方颗粒的计算值与实测值无显著性差异。结论 该方法可用于毛冬青配方颗粒中多指 标成分质量评价。 关键词：毛冬青；配方颗粒；绿原酸；酚酸；高效液相色谱法；一测多评法；相对校正因子 中图分类号：R917.101 文献标志码：B 文章编号：1007-7693(2017)08-1118-04 DOI: 10.13748/ki.issn1007-7693.2017.08.010 引用本文：农新维, 梁冰丽, 李颖, 等. 壮药毛冬青配方颗粒一测多评的定量研究[J]. 中国现代应用药学, 2017, 34(8): 1118-1121. Quantitative Analysis of Zhuang Medicine Ilex Pubescens Formula Granules by QAMS * NONG Xinwei, LIANG Bingli, LI Ying, HUANG Xiongmei, WEI Hongyan (Pura Pharm (Nanning) Pharmaceutical Company Limited, Nanning 530007, China) ABSTRACT: OBJECTIVE To establish a method for simultaneous detemination of six phenolic acids in Ilex pubescens formula granules by quantitative analysis of multi-components by single marker (QAMS). METHODS Chlorogenic acid was used as the internal reference substance. The relative correction factors (RCF) of chlorogenic acid, cryptochlorogenic acid, caffeic acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C were established respectively, and their contents in samples were calculated by RCF. At the same time, the external standard method(ESM) was used to detemine the above six phenolic acids. Compared with the content results determined by the ESM and QAMS, the accuracy and feasibility of QAMS method were verified. RESULTS The reproducibility of RCF was acceptable. There was no significant difference between the quantitative result